Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system

Hayato M Yamanouchi; Azusa Kamikouchi; Ryoya Tanaka

doi:10.1016/j.xpro.2023.102623

Protocol to investigate the neural basis for copulation posture of Drosophila using a closed-loop real-time optogenetic system

STAR Protoc. 2023 Dec 15;4(4):102623. doi: 10.1016/j.xpro.2023.102623. Epub 2023 Oct 2.

Authors

Hayato M Yamanouchi¹, Azusa Kamikouchi², Ryoya Tanaka³

Affiliations

¹ Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan. Electronic address: haya.m.yamano.neuro@gmail.com.
² Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya, Aichi 464-8602, Japan; Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601, Japan.
³ Graduate School of Science, Nagoya University, Nagoya, Aichi 464-8602, Japan; Institute for Advanced Research, Nagoya University, Nagoya, Aichi 464-8601, Japan. Electronic address: tanaka.ryoya.z3@f.mail.nagoya-u.ac.jp.

Abstract

In internal fertilization animals, maintaining a copulation posture facilitates the process of transporting gametes from male to female. Here, we present a protocol to investigate the neural basis for copulation posture of fruit flies using a closed-loop real-time optogenetic system. We describe steps for using deep learning analysis to enable optogenetic manipulation of neural activity only during copulation with high efficiency. This system can be applied to various animal behaviors other than copulation. For complete details on the use and execution of this protocol, please refer to Yamanouchi et al. (2023).¹.

Keywords: Bioinformatics; Model Organisms; Neuroscience; Systems Biology.

MeSH terms

Animals
Behavior, Animal
Computer Systems
Copulation*
Drosophila*
Female
Male
Optogenetics / methods