Development and validation of real-time recombinase polymerase amplification-based assays for detecting HPV16 and HPV18 DNA

Microbiol Spectr. 2023 Dec 12;11(6):e0120723. doi: 10.1128/spectrum.01207-23. Epub 2023 Oct 3.

Abstract

HPV DNA screening is an effective approach for the prevention of cervical cancer. The novel real-time recombinase polymerase amplification-based HPV detection systems we developed constitute an improvement over the HPV detection methods currently used in clinical practice and should help to extend cervical cancer screening in the future, particularly in point-of-care test settings.

Keywords: cervical cancer; human papillomavirus; real-time recombinase polymerase amplification.

MeSH terms

  • DNA, Viral / genetics
  • Early Detection of Cancer / methods
  • Female
  • Human papillomavirus 16 / genetics
  • Human papillomavirus 18 / genetics
  • Humans
  • Papillomavirus Infections* / diagnosis
  • Recombinases
  • Uterine Cervical Neoplasms* / diagnosis

Substances

  • Recombinases
  • DNA, Viral