Improvement of the mismatch amplification mutation assay-PCR for discrimination between Streptococcus suis serotypes 2 and 1/2

Erina Okuhama-Yoshida; Mizuki Nakayama; Miki Hattori; Daisuke Takamatsu; Masatoshi Okura

doi:10.1016/j.mimet.2023.106828

Improvement of the mismatch amplification mutation assay-PCR for discrimination between Streptococcus suis serotypes 2 and 1/2

J Microbiol Methods. 2023 Nov:214:106828. doi: 10.1016/j.mimet.2023.106828. Epub 2023 Sep 30.

Authors

Erina Okuhama-Yoshida¹, Mizuki Nakayama¹, Miki Hattori², Daisuke Takamatsu³, Masatoshi Okura⁴

Affiliations

¹ Miyazaki Prefectural Livestock Hygiene Service Center, 3151-1 Shimonaka, Sadoharacho, Miyazaki 880-0212, Japan.
² Fukuoka Prefectural Chuo Livestock Hygiene Service Center, 4-14-5 hakozakifutou, Higashi-ku, Fukuoka 812-0051, Japan.
³ Division of Infectious Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan; Joint Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagito, Gifu 501-1193, Japan; The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagito, Gifu 501-1193, Japan.
⁴ Division of Transboundary Animal Disease Research, Kagoshima Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 702 Chuzan, Kagoshima 891-0105, Japan. Electronic address: mokura@affrc.go.jp.

PMID: 37783232
DOI: 10.1016/j.mimet.2023.106828

Abstract

A mismatch amplification mutation assay (MAMA)-PCR, which detects a single-nucleotide polymorphism contributed to serological difference between Streptococcus suis serotypes 2 and 1/2, is used to discriminate between these serotypes. The present study reports unusual serotype 1/2 isolates untypable by the MAMA-PCR and improvement of the MAMA-PCR for typing such isolates.

Keywords: Amino acid substitution; MAMA-PCR; Serotyping; Streptococcus suis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Mutation
Polymerase Chain Reaction
Serogroup
Serotyping
Streptococcal Infections* / diagnosis
Streptococcus suis* / genetics
Swine
Swine Diseases* / diagnosis