Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey

Xiao-Hua Zhang; Xiang-Dong Qing; Jing-Jing Zheng; Yan Yu; Jiaojiao Huang; Chao Kang; Zhi Liu

doi:10.1016/j.fochx.2023.100766

Aqueous two-phase systems coupled with chemometrics-enhanced HPLC-DAD for simultaneous extraction and determination of flavonoids in honey

Food Chem X. 2023 Jun 21:19:100766. doi: 10.1016/j.fochx.2023.100766. eCollection 2023 Oct 30.

Authors

Xiao-Hua Zhang^{1

2}, Xiang-Dong Qing³, Jing-Jing Zheng², Yan Yu², Jiaojiao Huang⁴, Chao Kang⁵, Zhi Liu⁴

Affiliations

¹ Department of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang, Hunan 414006, China.
² Henan Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety, Food and Pharmacy College, Xuchang University, Xuchang, China.
³ Hunan Provincial Key Laboratory of Dark Tea and Jin-hua, College of Materials and Chemical Engineering, Hunan City University, Yiyang, China.
⁴ College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi, China.
⁵ School of Chemistry and Chemical Engineering, Guizhou University, Guiyang, China.

Abstract

In this study, an accurate, rapid, green, and environment friendly method for the extraction and quantitative analysis of flavonoids in honey was established by using the aqueous two-phase extraction combined with the chemometrics-assisted HPLC-DAD. The first purpose of this study was to extract seven flavonoids in five different types of honey using alcohol/salt aqueous two-phase system (ATPS). The system with 2.82 mL sodium citrate (30%), 1.58 mL water, and 3.10 mL isopropanol, showed the highest flavonoids extraction yields in the top phase (87.66-101.50%). Additionally, the three-way array of honey samples based on HPLC-DAD was decomposed mathematically by the alternating trilinear decomposition (ATLD) algorithm to obtain reasonable chromatograms, spectra, and concentration profiles for each analyte. Compared with the traditional solid-phase extraction method, the ATPS-ATLD-based method showed satisfactory spiked recoveries, lower limit of detection, and higher sensitivity, further verifying its accuracy and stability.

Keywords: Aqueous two-phase systems; Chemometrics; Flavonoids; HPLC-DAD; Honey.