Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine

Lokman Liv; Merve Portakal; Meryem Sıla Çukur; Beyza Topaçlı; Berkay Uzun

doi:10.1021/acsomega.3c02561

Electrocatalytic Determination of Uric Acid with the Poly(Tartrazine)-Modified Pencil Graphite Electrode in Human Serum and Artificial Urine

ACS Omega. 2023 Sep 11;8(38):34420-34430. doi: 10.1021/acsomega.3c02561. eCollection 2023 Sep 26.

Authors

Lokman Liv¹, Merve Portakal^{1

2}, Meryem Sıla Çukur^{1

3}, Beyza Topaçlı^{1

4}, Berkay Uzun^{1

3}

Affiliations

¹ Electrochemistry Laboratory, Chemistry Group, The Scientific and Technological Research Council of Turkey, National Metrology Institute, (TUBITAK UME), 41470 Gebze, Kocaeli, Turkey.
² Faculty of Technology, Department of Biomedical Engineering, Pamukkale University, 20160 Denizli, Turkey.
³ Faculty of Technology, Department of Biomedical Engineering, Kocaeli University, İzmit, 41380 Kocaeli, Turkey.
⁴ School of Engineering, Department of Biomedical Engineering, TOBB University of Economics and Technology, 06560 Ankara, Turkey.

Abstract

A novel electrocatalytic sensing strategy was built for uric acid (UA) determination with an exceptionally developed poly(tartrazine)-modified activated pencil graphite electrode (pTRT/aPGE) in human serum and artificial urine. The oxidation signal of UA at 275 mV in pH 7.5 phosphate buffer solution served as the analytical response. Cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy were used to characterize the sensing platform, which was able to detect 0.10 μM of UA in the ranges of 0.34-60 and 70-140 μM. The samples of human serum and artificial urine were analyzed by both the pTRT/aPGE and the uricase-modified screen-printed electrode. The results were statistically evaluated and compared with each other within the confidence level of 95%, and no significant difference between the results was found.