miR-22a targets p62/SQSTM1 to negatively affect autophagy and disease resistance of grass carp (Ctenopharyngodon idella)

Hongyan Yu; Lingli Xie; Zheyan Chen; Huiqin Niu; Xuewen Jia; Biao Du; Yubang Shen; Lang Gui; Xiaoyan Xu; Jiale Li

doi:10.1016/j.fsi.2023.109124

miR-22a targets p62/SQSTM1 to negatively affect autophagy and disease resistance of grass carp (Ctenopharyngodon idella)

Fish Shellfish Immunol. 2023 Nov:142:109124. doi: 10.1016/j.fsi.2023.109124. Epub 2023 Sep 28.

Authors

Hongyan Yu¹, Lingli Xie¹, Zheyan Chen¹, Huiqin Niu¹, Xuewen Jia¹, Biao Du¹, Yubang Shen², Lang Gui², Xiaoyan Xu³, Jiale Li⁴

Affiliations

¹ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
² Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
³ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: xyxu@shou.edu.cn.
⁴ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: jlli@shou.edu.cn.

PMID: 37777097
DOI: 10.1016/j.fsi.2023.109124

Abstract

MicroRNAs (miRNAs) are integral to many biological functions, including autophagy, a process recently proven to be closely linked to innate immunity. In this study, we present findings on miR-22a, a teleost homolog of mammalian miR-22, illustrating its capacity to target the autophagy adaptor p62, subsequently inducing downregulation at both mRNA and protein levels. Utilizing Western blot analyses, we demonstrated that miR-22a inhibits the autophagy flux of CIK cells, correlated with an elevated presence of LC3 II. Additionally, the overexpression of miR-22a resulted in the suppression of NF-κB signaling, leading to reduced cellar antimicrobial abilities and increased apoptosis. These findings provide novel insights into the role of miR-22a as an autophagy-related miRNA and its immune mechanisms against pathogens via p62 in teleost, enriching our understanding of the interplay between autophagy and innate immunity.

Keywords: Autophagy; Grass carp; Immune; miR-22a.

MeSH terms

Animals
Autophagy
Carps* / genetics
Carps* / metabolism
Disease Resistance
Fish Diseases*
Fish Proteins
Immunity, Innate / genetics
Mammals / metabolism
MicroRNAs* / genetics
Sequestosome-1 Protein / metabolism

Substances

Sequestosome-1 Protein
MicroRNAs
Fish Proteins