Cancer Cell Silicification and Surface Functionalization to Create Microbial Mimetic Cancer Vaccines

Abstract

As cancer progresses, tumor cells adapt to evade immune cells. To counter this, cancer cells can be silicified ex vivo, creating surface masks that can be decorated with microbial-associated molecules that are readily recognized by antigen-presenting cells (APCs). The transformation process renders the tumor cells nonviable and preserves the integrity of the cell and associated tumor antigens. The resulting personalized cancer vaccine, when returned to the patient, engages molecules on the surface of APC, activating signaling pathways that lead to immune cell activation, vaccine internalization, processing of tumor antigens, and major histocompatibility complex peptide presentation to T cells. The cancer-specific T cells then circulate throughout the body, killing tumor cells. This chapter presents detailed methods for the cryogenic precipitation of silica on cellular structures (cryo-silicification), creating vaccines that are potent immune activators. Further, silicified cells can be dehydrated for shelf storage, eliminating the need for costly cryogenic storage.

Keywords: Biomineralization; Cancer; CpG oligodeoxyribonucleotide; Dehydration; Monophosphoryl lipid A; Ovarian; Polyethyleneimine; Silicification; Toll-like receptor ligand; Vaccine.