Fluviispira vulneris sp. nov., isolated from human wound secretions

Antonie Van Leeuwenhoek. 2023 Dec;116(12):1305-1316. doi: 10.1007/s10482-023-01883-4. Epub 2023 Sep 29.

Abstract

Human infections by environmental bacteria is becoming an increasing problem and has become a matter of great concern due to the adverse effects worldwide. In this study, we reported a new environmental pathogen. Isolate GX5518T was a novel Gram-negative, aerobic, non-motile, pleomorphic and red-pigmented bacterium, was isolated from human wound secretions (GuangXi, People's Republic of China). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 °C (optimum, 28-32 °C) with 0-1.5% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that isolate GX5518T was closely related to Fluviispira sanaruensis JCM 31447T (99.73%) and Fluviispira multicolorata 33A1-SZDPT (98.49%). However, the estimated ANI values of the isolate GX5518T compared to the F. sanaruensis JCM 31447T and F. multicolorata 33A1-SZDPT were 88.67% and 77.35%, respectively. The estimated dDDH, ANI and AAI values between isolate GX5518T and its closely related strains were below the threshold values generally considered for recognizing a new species. The genome size was 3.6 Mbp and the DNA G + C content was 33.1%. The predominant fatty acids (> 5%) in GX5518T cells were iso-C15:0, C16:0, C17:0, C17:1 ω8c and C16:1 ω7c/C16:1 ω6c. The major menaquinone was MK-8 (86.9%). The polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and three unknown lipids (L1-3). The chemical composition was different from that of the F. sanaruensis JCM 31447T. Comparative genomics analysis between isolate GX5518T and its related strains revealed that there were a number of genes involved in resistance to antibiotics and toxic compounds in isolate GX5518T, which were responsible for the copper homeostasis, cobalt-zinc-cadmium resistance, resistance to fluoroquinolones, and zinc resistance. Based on the phenotypic, chemotaxonomic, and genomic analyses, isolate GX5518T (= CGMCC 1.18685T = KCTC 82149T) represents a novel species of the genus Fluviispira, for which the name Fluviispira vulneris sp. nov. is proposed.

Keywords: 16S rRNA; Fluviispira vulneris sp. nov.; Polyphasic taxonomy; Wound infections.

MeSH terms

  • Bacterial Typing Techniques
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids* / chemistry
  • Humans
  • Nucleic Acid Hybridization
  • Phospholipids* / chemistry
  • Phylogeny
  • Proteobacteria / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Zinc

Substances

  • Phospholipids
  • RNA, Ribosomal, 16S
  • DNA, Bacterial
  • Fatty Acids
  • Zinc