Identification and characterization of the Bicupin domain family and functional analysis of GhBCD11 in response to verticillium wilt in cotton

Ying Sun; Yi Wang; Yalin Zhang; Nadeem Hasan; Na Yang; Yijing Xie; Canming Tang

doi:10.1016/j.plantsci.2023.111875

Identification and characterization of the Bicupin domain family and functional analysis of GhBCD11 in response to verticillium wilt in cotton

Plant Sci. 2023 Sep 27:337:111875. doi: 10.1016/j.plantsci.2023.111875. Online ahead of print.

Authors

Ying Sun¹, Yi Wang¹, Yalin Zhang², Nadeem Hasan¹, Na Yang¹, Yijing Xie¹, Canming Tang³

Affiliations

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
² State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, Henan, China.
³ State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China. Electronic address: tangcm@njau.edu.cn.

PMID: 37769874
DOI: 10.1016/j.plantsci.2023.111875

Abstract

Bicupin domain protein (BCD) family, an important component of Cupin domain superfamily, plays important roles in oxalic acid (OA) degradation and stress responses in high plants. However, no studies have been reported on the Cupin domain family in cotton up till now. In our study, a total 110 proteins including Cupin domain were identified from the upland cotton (Gossypium hirsutum). Among them, 17 proteins contained Bicupin domain. Subsequently, we found that V. dahliae produces OA leading to cotton leaf wilting. RT-qPCR analysis of GhBCDs revealed that OA and V. dahliae Vd080 significantly enhanced the expression of GhBCD11. The Virus-induced gene silencing and overexpression analysis showed that GhBCD11 positively regulates plant resistance to V. dahliae. Subcellular localization showed GhBCD11 located on the plasma membrane. The analysis of expression pattern showed that GhBCD11 can be induced via hormone-mediated signal pathway including salicylic acid (SA), ethephon (ET), methyl jasmonate (JA) and abscisic acid (ABA). In addition, we identified an interaction between 60 S ribosomal protein GhRPL12-3 and GhBCD11 by yeast double hybridization. Overall, this is the first study, where we identified Cupin domain family in cotton, clarified the role of GhBCD11 in cotton for resistance to V. dahliae and found an interaction between GhRPL12-3 and GhBCD11.

Keywords: Bicupin gen family; Cotton; GhBCD11; GhRPL12–3; Verticillium wilt.