The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway

Huiting Wang; Xiaoman Zheng; Yongshi Lin; Xirui Zheng; Mingen Yan; Yaoxing Li; Dayou Shi; Shining Guo; Cui Liu

doi:10.1016/j.psj.2023.103032

The mixture of Radix isatidis, Forsythiae, and Gypsum alleviates lipopolysaccharide-induced fever in broilers by inhibition of TLR4/NF-κB signaling pathway

Poult Sci. 2023 Nov;102(11):103032. doi: 10.1016/j.psj.2023.103032. Epub 2023 Aug 18.

Authors

Huiting Wang¹, Xiaoman Zheng¹, Yongshi Lin¹, Xirui Zheng¹, Mingen Yan¹, Yaoxing Li¹, Dayou Shi², Shining Guo², Cui Liu³

Affiliations

¹ College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
² College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou, 510642, China; International Institute of Traditional Chinese Veterinary Medicine, Guangzhou, 510642, China.
³ College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China. Electronic address: liuc@scau.edu.cn.

Abstract

To determine whether the antipyretic effect of the mixture of Radix isatidis, Forsythiae, and Gypsum (RIFG) on lipopolysaccharide (LPS) induced fever broilers and its related mechanisms. A total of 315 24-day-old yellow-plumed broilers were randomly divided into 7 groups, except for the control group, other groups were injected with LPS. Two hours later, RIFG were given drinking water to relieve fever, and it was evaluated by the expression of genes and proteins of the maximum body temperature rise (∆T), body temperature response index (TRI), serum and hypothalamic pyrogenic heat factor. RIFG could reduce the body temperature of broilers with fever (P < 0.01). It inhibited the expressions of IL-6 and PGE2 (P < 0.01), down-regulated mRNA expression levels of TNF-ɑ and COX-2 (P < 0.01), and promoted the generation of antipyretic factor AVP mRNA (P < 0.01). In addition, the expression level of TLR4 and NF-κB p65 protein can be down-regulated, and LPS + RM group has the best down-regulated effect. RIFG had a good antipyretic effect on reducing LPS-induced fever of broilers by inhibiting the activation of TLR4/NF-κB signaling pathway and thermogenic factors.

Keywords: TLR4/NF-κB signaling pathway; antipyretic effect; broiler; lipopolysaccharide.