General requirements for the production of extracellular vesicles derived from human stem cells

Ling Wang; Shiyu Liu; Ka Li; Aijin Ma; Chenghu Hu; Changlin Wang; Nan Cao; Yunpeng Zhao; Ruifeng Fu; Wenwen Jia; Peng Xiang; Houqi Liu; Zhongquan Qi; Ningwen Zhu; Lingmin Liang; Lei Wang; Jiani Cao; Peijun Zhai; Jiaxi Zhou; Jun Wei; Tao Na; Jun Wu; Zhiying He; Guangdong Zhou; Weifeng Yu; Jinyan Wu; Wen Zeng; Yong Zhang; Lijun Zhu; Boqiang Fu; Jingzhong Zhang; Shuwei Yang; Chengxiang Dai; Hengmi Cui; Jianzhong Jing; Hexin Yan; Xiaowen He; Yongbo Lu; Cailing Tong; Tongbiao Zhao; Jie Hao; Xialin Liu; Yan Jin; Yue Wang

doi:10.1111/cpr.13554

General requirements for the production of extracellular vesicles derived from human stem cells

Cell Prolif. 2024 Mar;57(3):e13554. doi: 10.1111/cpr.13554. Epub 2023 Sep 28.

Authors

Ling Wang^{1

2}, Shiyu Liu³, Ka Li⁴, Aijin Ma^{5

6}, Chenghu Hu⁷, Changlin Wang⁸, Nan Cao^{6

9}, Yunpeng Zhao^{1

2}, Ruifeng Fu^{1

2}, Wenwen Jia^{10

11}, Peng Xiang^{6

9}, Houqi Liu¹², Zhongquan Qi¹³, Ningwen Zhu¹⁴, Lingmin Liang^{6

15

16}, Lei Wang^{6

15

16}, Jiani Cao^{6

15

16}, Peijun Zhai¹⁷, Jiaxi Zhou^{6

18}, Jun Wei^{6

19}, Tao Na²⁰, Jun Wu^{15

16}, Zhiying He²¹, Guangdong Zhou²², Weifeng Yu²², Jinyan Wu¹⁴, Wen Zeng²³, Yong Zhang^{6

24

25}, Lijun Zhu²⁶, Boqiang Fu^{6

27}, Jingzhong Zhang²⁸, Shuwei Yang²⁹, Chengxiang Dai³⁰, Hengmi Cui³¹, Jianzhong Jing³², Hexin Yan³³, Xiaowen He³⁴, Yongbo Lu³⁵, Cailing Tong³⁶, Tongbiao Zhao^{6

15

16}, Jie Hao^{6

15

16}, Xialin Liu³⁷, Yan Jin³, Yue Wang^{1

2

11}

Affiliations

¹ Department of Stem Cells and Regenerative Medicine, Center for Translational Medicine, Naval Medical University, Shanghai, China.
² Shanghai Key Laboratory of Cell Engineering, Naval Medical University, Shanghai, China.
³ State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Shaanxi, China.
⁴ Institute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, China.
⁵ Beijing Technology and Business University, Beijing, China.
⁶ Chinese Society for Stem Cell Research, Shanghai, China.
⁷ Xi'an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research, Northwestern Polytechnical University, Shaanxi, China.
⁸ China Institute of Standardization, Beijing, China.
⁹ Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, China.
¹⁰ National Stem Cell Resource Transformation Bank, Shanghai, China.
¹¹ Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China.
¹² Haimenshengyuan Stem Cell Technology Co. Ltd., Nantong, China.
¹³ Guangxi University, Nanning, China.
¹⁴ Fudan University, Shanghai, China.
¹⁵ National Stem Cell Resource Center, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Institute for Stem Cell and Regeneration, University of Chinese Academy of Sciences, Beijing, China.
¹⁶ Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
¹⁷ China National Accreditation Center for Conformity Assessment, Beijing, China.
¹⁸ Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
¹⁹ Zephyrm Biotechnologies Co. Ltd., Beijing, China.
²⁰ China Institute for Food and Drug Control, Beijing, China.
²¹ Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China; Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China.
²² Shanghai Jiaotong University, Shanghai, China.
²³ Army Military Medical University, Chongqing, China.
²⁴ National Clinical Research Center of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
²⁵ HHLIFE Company Inc., Shenzhen, China.
²⁶ Institute of Scientific and Technical Information of China, Beijing, China.
²⁷ National Institute of Metrology, Beijing, China.
²⁸ Suzhou Institute of Biomedical Engineering Technology, Chinese Academy of Sciences, Suzhou, China.
²⁹ Guangzhou Funeng Gene Co. Ltd., Guangzhou, China.
³⁰ Cellular Biomedicine Group Ltd, Shanghai, China.
³¹ Jiangsu Innovation Institute For Biomedicine, Nanjing, China.
³² Shanghai Golden Meditech LifeBank Co. Ltd., Shanghai, China.
³³ Shanghai Celevik Biotechnology Co. Ltd., Shanghai, China.
³⁴ Origincell Co. Ltd., Shanghai, China.
³⁵ Guangdong Boxi Biotechnology Co. Ltd., Dongguan, China.
³⁶ Biotechcomer (Xiamen) Technology Co. Ltd., Xiamen, China.
³⁷ State Key Laboratory of Ophthalmology, Zhongshan ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Abstract

'General requirements for the production of extracellular vesicles derived from human stem cells' is the first guideline for stem cells derived extracellular vesicles in China, jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research. This standard specifies the general requirements, process requirements, packaging and labelling requirements and storage requirements for preparing extracellular vesicles derived from human stem cells, which is applicable to the research and production of extracellular vesicles derived from stem cells. It was originally released by the China Society for Cell Biology on 30 August 2022. We hope that the publication of this guideline will promote institutional establishment, acceptance and execution of proper protocols, and accelerate the international standardisation of extracellular vesicles derived from human stem cells.

MeSH terms

China
Extracellular Vesicles*
Humans
Stem Cells*

Abstract

MeSH terms

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