Purification and characterization of 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase involved in ergothioneine utilization in Burkholderia sp. HME13

Hisashi Muramatsu; Daisuke Inouchi; Masaaki Yamada; Akihito Koujitani; Hiroki Maguchi; Shin-Ichiro Kato

doi:10.1093/bbb/zbad139

Purification and characterization of 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase involved in ergothioneine utilization in Burkholderia sp. HME13

Biosci Biotechnol Biochem. 2023 Dec 19;88(1):74-78. doi: 10.1093/bbb/zbad139.

Authors

Hisashi Muramatsu¹, Daisuke Inouchi², Masaaki Yamada³, Akihito Koujitani³, Hiroki Maguchi³, Shin-Ichiro Kato¹

Affiliations

¹ Multidisciplinary Science Cluster, Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi, Japan.
² Faculty of Agriculture and Marine Science, Kochi University, B200 Monobe, Nankoku, Kochi, Japan.
³ Graduate School of Integrated Arts and Sciences, Kochi University, B200 Monobe, Nankoku, Kochi, Japan.

PMID: 37766390
DOI: 10.1093/bbb/zbad139

Abstract

Recombinant 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase (ErtC) derived from Burkholderia sp. HME13 was purified to homogeneity. Here, ErtC's kinetic parameters, optimum reaction temperature and pH, and stability at varying temperatures and pH and the effects of various additives on ErtC activity were determined. Real-time polymerase chain reaction and enzyme assays suggested that ergothioneine induced the expression of ertC.

Keywords: Burkholderia; 3-(2,5-dioxoimidazolidin-4-yl) propionic acid; 3-(5-oxo-2-thioxoimidazolidin-4-yl) propionic acid desulfhydrase; DUF917 protein family; ergothioneine.

MeSH terms

Burkholderia*
Ergothioneine*
Propionates
Temperature

Substances

propionic acid
Ergothioneine
Propionates

Grants and funding

21K07005/JSPS