A Bacillus subtilis Strain ZJ20 with AFB1 Detoxification Ability: A Comprehensive Analysis

Meixue Huang; Jing Guo; Yanyan Jia; Chengshui Liao; Lei He; Jing Li; Ying Wei; Songbiao Chen; Jian Chen; Ke Shang; Rongxian Guo; Ke Ding; Zuhua Yu

doi:10.3390/biology12091195

A Bacillus subtilis Strain ZJ20 with AFB1 Detoxification Ability: A Comprehensive Analysis

Biology (Basel). 2023 Aug 31;12(9):1195. doi: 10.3390/biology12091195.

Authors

Meixue Huang^{1

2}, Jing Guo^{1

2}, Yanyan Jia^{1

2}, Chengshui Liao^{1

2}, Lei He^{1

2}, Jing Li^{1

2}, Ying Wei^{1

2}, Songbiao Chen^{1

2}, Jian Chen^{1

2}, Ke Shang^{1

2}, Rongxian Guo^{1

2}, Ke Ding^{1

2}, Zuhua Yu^{1

2}

Affiliations

¹ Luoyang Key Laboratory of Live Carrier Biomaterial and Animal Disease Prevention and Control, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
² Laboratory of Functional Microbiology and Animal Health, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.

Abstract

As a class I carcinogen, aflatoxin can cause serious damage to various tissues and organs through oxidative stress injuries. The liver, as the target organ of AFB₁, is the most seriously damaged. Biological methods are commonly used to degrade AFB₁. In our study, the aflatoxin B₁-degrading strain ZJ20 was screened from AFB₁-contaminated feed and soil, and the degradation of AFB₁ by ZJ20 was investigated. The whole genome of strain ZJ20 was analyzed, revealing the genomic complexity of strain ZJ20. The 16S rRNA analysis of strain ZJ20 showed 100% identity to Bacillus subtilis IAM 12118. Through whole gene functional annotation, it was determined that ZJ20 has high antioxidant activity and enzymatic activity; more than 100 CAZymes and 11 gene clusters are involved in the production of secondary metabolites with antimicrobial properties. In addition, B. subtilis ZJ20 was predicted to contain a cluster of genes encoding AFB₁-degrading enzymes, including chitinase, laccase, lactonase, and manganese oxidase. The comprehensive analysis of B. subtilis provides a theoretical basis for the subsequent development of the biological functions of ZJ20 and the combinatorial enzyme degradation of AFB₁.

Keywords: AFB1; Aflatoxin-degrading enzyme; Bacillus subtilis; CAZymes; secondary metabolites; whole genome sequence.

Grants and funding

nos. 32072771 and no. 182300410052/National Natural Science Foundation of China (grant nos. 32072771) and Natural Science Foundation of Henan Province (grant no. 182300410052)