Fabrication of a magnetic metal-organic framework/covalent organic framework composite for simultaneous magnetic solid-phase extraction of seventeen trace quinolones residues in meats

Shumin Lin; Bolong Liang; Zhe Zhao; Zhenqiu Li; Kai Deng; Hongbin He; Shu-Xuan Liang

doi:10.1016/j.chroma.2023.464403

Fabrication of a magnetic metal-organic framework/covalent organic framework composite for simultaneous magnetic solid-phase extraction of seventeen trace quinolones residues in meats

J Chromatogr A. 2023 Oct 25:1709:464403. doi: 10.1016/j.chroma.2023.464403. Epub 2023 Sep 20.

Authors

Shumin Lin¹, Bolong Liang², Zhe Zhao³, Zhenqiu Li⁴, Kai Deng⁵, Hongbin He², Shu-Xuan Liang⁶

Affiliations

¹ Key Laboratory of Analytical Science and Technology of Hebei Province, State Key Laboratory of New Pharmaceutical Preparations and Excipients, College of Chemistry and Materials Science, Hebei University, Baoding 071002, PR China; Analysis and Testing Center, Inner Mongolia University of Science and Technology, Baotou 014010, China.
² School of Eco-Environment, Hebei University, Baoding 071002, PR China.
³ Key Laboratory of Analytical Science and Technology of Hebei Province, State Key Laboratory of New Pharmaceutical Preparations and Excipients, College of Chemistry and Materials Science, Hebei University, Baoding 071002, PR China.
⁴ College of Life Science, Hebei University, Baoding 071002, PR China; Institute of Life Science and Green Development, Hebei University, Baoding 071002, PR China.
⁵ College of Life Science, Hebei University, Baoding 071002, PR China.
⁶ Key Laboratory of Analytical Science and Technology of Hebei Province, State Key Laboratory of New Pharmaceutical Preparations and Excipients, College of Chemistry and Materials Science, Hebei University, Baoding 071002, PR China; Institute of Life Science and Green Development, Hebei University, Baoding 071002, PR China. Electronic address: liangsx168@126.com.

PMID: 37757609
DOI: 10.1016/j.chroma.2023.464403

Abstract

Effective capture of quinolones (QNs) in animal-derived food is a vital procedure for food safety monitoring. However, the lack of adsorption specificity and difficult to recycle in complex substrate conditions have been major problems for most of the adsorbents. In this work, a magnetic Fe₃O₄/MOF/COF composite (named Fe₃O₄@NH₂-MIL-125@TpPa-SO₃H) was successfully synthesized with good magnetic responsiveness and conspicuous affinity towards QNs. The Fe₃O₄/MOF/COF composite was used as a magnetic solid-phase extraction (MSPE) adsorbent for pretreatment and determination of QNs in meat samples. Under optimal MSPE conditions in combination with high performance liquid chromatography-quadrupole orbitrap high resolution mass spectrometer (HPLC-Q-Orbitrap HRMS), the proposed method had good linearity (R² ≥ 0.9978) from 0.01 to 100ng g^-1, low limits of detection (0.0016 to 0.0940ng g^-1), good precision with relative standard deviations lower than 5.8%. This method was effectively applied to the detection of 17 QNs in the spiked pork, chicken and beef samples with satisfactory recoveries from 83.9 to 106.2%. The separation selectivity mainly due to the π-π interaction, hydrogen bonding, and electrostatic attraction between QNs and the sulfonic acid and amino functional groups of the composite. After verification, the stability and reusability of the composite meet the requirements of complex matrix sample pretreatment. The developed MSPE method based on the magnetic Fe₃O₄/MOF/COF composite provided an ideal sample pretreatment alternative for determining trace QNs in complex matrixes with selectivity, simplicity, rapidity, and efficiency.

Keywords: Covalent organic frameworks; HPLC-Q-orbitrap mass spectrometry; Magnetic solid-phase extraction; Metal-organic frameworks; Quinolones residues.