Bioactive compounds from Vellozia pyrantha A.A.Conc: A metabolomics and multivariate statistical analysis approach

Luiz A F Ribeiro; Iago B F Dos Santos; Caline G Ferraz; Lourdes C de Souza-Neta; Valdenizia R Silva; Luciano de S Santos; Daniel P Bezerra; Milena B P Soares; Leonardo Zambotti-Villela; Pio Colepicolo; Antonio G Ferreira; Floricéa M Araújo; Paulo R Ribeiro

doi:10.1016/j.fitote.2023.105686

Bioactive compounds from Vellozia pyrantha A.A.Conc: A metabolomics and multivariate statistical analysis approach

Fitoterapia. 2023 Dec:171:105686. doi: 10.1016/j.fitote.2023.105686. Epub 2023 Sep 23.

Affiliations

¹ Metabolomics Research Group, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil; Programa de Pós-Graduação em Química Aplicada (PGQA), Universidade do Estado da Bahia, Salvador, Brazil; Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil.
² Metabolomics Research Group, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil; Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil.
³ Programa de Pós-Graduação em Química Aplicada (PGQA), Universidade do Estado da Bahia, Salvador, Brazil.
⁴ Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil.
⁵ Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
⁶ Laboratório de Ressonância Magnética Nuclear, Universidade Federal de São Carlos, São Carlos, Brazil.
⁷ Metabolomics Research Group, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil.
⁸ Metabolomics Research Group, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil; Programa de Pós-Graduação em Química Aplicada (PGQA), Universidade do Estado da Bahia, Salvador, Brazil; Programa de Pós-Graduação em Química, Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil. Electronic address: paulodc3@gmail.com.

PMID: 37748714
DOI: 10.1016/j.fitote.2023.105686

Abstract

The chemical composition of V. pyrantha resin (VpR) and fractions (VpFr1-7 and VpWS) were assessed by LC-MS and NMR. Twenty-eight metabolites were identified, including 16 diterpenoids, seven nor-diterpenoids, one fatty acid, one bis-diterpenoid, one steroid, one flavonoid, and one triterpenoid. The pharmacological potential of VpR, VpFr1-7, and isolated compounds was assessed by determining their antioxidant, antimicrobial, and cytotoxic activities. VpFr4 (IC₅₀ = 205.48 ± 3.37 μg.mL^-1) had the highest antioxidant activity, whereas VpFr6 (IC₅₀ = 842.79 ± 10.23 μg.mL^-1) had the lowest. The resin was only active against Staphylococcus aureus (MIC 62.5 μg.mL^-1) and Salmonella choleraesius (MIC and MFC 500 μg.mL^-1), but fractions were enriched with antibacterial compounds. V. pyrantha resin and fractions showed great cytotoxic activity against HCT116 (IC₅₀ = 20.08 μg.mL^-1), HepG2 (IC₅₀ = 20.50 μg.mL^-1), and B16-F10 (12.17 μg.mL^-1) cell lines. Multivariate statistical analysis was used as a powerful tool to pinpoint possible metabolites responsible for the observed activities.

Keywords: Biological activity; Cleistanthane; Diterpenoids; Metabolomics; Multivariate statistical analysis.

MeSH terms

Anti-Bacterial Agents / pharmacology
Anti-Infective Agents* / pharmacology
Antineoplastic Agents*
Antioxidants / chemistry
Antioxidants / pharmacology
Diterpenes* / pharmacology
Molecular Structure
Plant Extracts / chemistry

Substances

Plant Extracts
Anti-Infective Agents
Anti-Bacterial Agents
Antioxidants
Antineoplastic Agents
Diterpenes