Biofortification of different maize cultivars with zinc, iron and selenium by foliar fertilizer applications

Yan-Fang Xue; Xiao-Jing Li; Wei Yan; Qi Miao; Chun-Yan Zhang; Meng Huang; Jin-Bian Sun; Shi-Jun Qi; Zhao-Hua Ding; Zhen-Ling Cui

doi:10.3389/fpls.2023.1144514

Biofortification of different maize cultivars with zinc, iron and selenium by foliar fertilizer applications

Front Plant Sci. 2023 Sep 7:14:1144514. doi: 10.3389/fpls.2023.1144514. eCollection 2023.

Authors

Yan-Fang Xue^{1

2

3}, Xiao-Jing Li^{1

2

4}, Wei Yan^{1

2}, Qi Miao⁵, Chun-Yan Zhang⁶, Meng Huang^{1

2}, Jin-Bian Sun^{1

2

3}, Shi-Jun Qi^{1

2}, Zhao-Hua Ding^{1

2}, Zhen-Ling Cui^{1

2

5}

Affiliations

¹ National Engineering Research Center of Wheat and Maize, Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
² Key Laboratory of Biology and Genetic Improvement of Maize in Northern Yellow-Huai Rivers Plain, Ministry of Agriculture, Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
³ College of Agronomy, Liaocheng University, Liaocheng, China.
⁴ College of Life Sciences, Shandong Normal University, Jinan, China.
⁵ College of Resources and Environment, China Agricultural University, Beijing, China.
⁶ Food Crop Cultivation Institute, Linyi Academy of Agricultural Sciences, Linyi, China.

Abstract

Fertilizer-based biofortification is a strategy for combating worldwide malnutrition of zinc (Zn), iron (Fe) and selenium (Se). Field experiments were conducted to investigate the effects of foliar treatments on concentrations of Zn, Fe, Se, N and bioavailability of Zn and Fe in grains of three maize cultivars grown at three locations. We compared the efficacy of ZnO nanoparticles (ZnO-NPs), Zn complexed chitosan nanoparticles (Zn-CNPs), conventional ZnSO₄ and a cocktail solution (containing Zn, Fe and Se). All treatments were foliar-applied at rate of 452 mg Zn L^-1, plus urea. Applying ten-fold less Zn (at rate of 45.2 mg Zn L^-1) plus urea in the form of ZnO-NPs, Zn-CNPs, or ZnSO₄ resulted in no increase, or a negligible increase, in grain Zn concentration compared with deionized water. By contrast, among the different Zn sources plus urea applied by foliar sprays, conventional ZnSO₄ was the most efficient in improving grain Zn concentration. Furthermore, foliar application of a cocktail solution effectively improved grain concentrations of Zn, Fe, Se and N simultaneously, without a grain yield trade-off. For example, the average grain concentrations were simultaneously increased from 13.8 to 22.1 mg kg^-1 for Zn, from 17.2 to 22.1 mg kg^-1for Fe, from 21.4 to 413.5 ug kg^-1 for Se and from 13.8 to 14.7 g kg^-1 for N by foliar application of a cocktail solution. Because grain yield was significantly negatively correlated with grain nutrient concentrations, the magnitude of increase in grain concentrations of Zn and Fe was most pronounced in the maize cultivar with the lowest grain yield (Zhengdan958 grown in Linyi). Foliar application of a cocktail solution also significantly decreased the phytic acid (PA) concentration, ratios of PA/Fe and PA/Zn in grains, indicating an increased bioavailability of Fe and Zn for human health. In conclusion, we found that a foliar application of a cocktail solution including Zn, Fe, Se and N was most effective for biofortification, but that the grains with the lowest yield contained the greatest concentration of these elements. This finding highlights the need to breed maize varieties that are capable of achieving both high grain yield and high grain nutritional quality to address food security and human health challenges.

Keywords: ZnO nanoparticles; bioavailability; foliar application; iron; maize; selenium; zinc.

Grants and funding

This research was funded by National Key Research and Development Program of China (2021YFD1901003, 2017YFD0301005), the Innovative Talent Introduction and Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2023G23, CXGC2023A22), the project of Science and Technology commissioners in Shandong Province (2022DXAL0125), the Key Laboratory of Trace Element Nutrition, National Health Commission of the Peoples' Republic of China (wlkfz202318) and the Taishan Scholarship Project of Shandong Province (No.TS201712082).