Evaluation of antioxidant status and lipid peroxidation in dogs with myxomatous mitral valve degeneration stage B1

Katerina Tomsič; Aleksandra Domanjko Petrič; Ana Nemec; Tatjana Pirman; Vida Rezar; Alenka Seliškar; Tomaž Vovk; Alenka Nemec Svete

doi:10.3389/fvets.2023.1203480

Evaluation of antioxidant status and lipid peroxidation in dogs with myxomatous mitral valve degeneration stage B1

Front Vet Sci. 2023 Sep 6:10:1203480. doi: 10.3389/fvets.2023.1203480. eCollection 2023.

Authors

Katerina Tomsič¹, Aleksandra Domanjko Petrič¹, Ana Nemec¹, Tatjana Pirman², Vida Rezar², Alenka Seliškar¹, Tomaž Vovk³, Alenka Nemec Svete¹

Affiliations

¹ Small Animal Clinic, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia.
² Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Domžale, Slovenia.
³ The Chair of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia.

Abstract

Myxomatous mitral valve degeneration (MMVD) is the most common naturally occurring heart disease in dogs. There is a lack of data on antioxidant status and oxidative damage in dogs with MMVD stage B1 according to the American College of Veterinary Internal Medicine (ACVIM B1). The aim of this study was to investigate antioxidant status (plasma vitamin E, lipid-standardized vitamin E (LS-VitE), antioxidant capacity of lipid-(ACL) and water-soluble antioxidants, whole blood glutathione peroxidase and erythrocyte superoxide dismutase), and lipid peroxidation [malondialdehyde (MDA)] in dogs with MMVD ACVIM B1. Serum cholesterol and triglyceride concentrations were measured to calculate LS-VitE. Fourteen dogs with MMVD ACVIM B1 and 12 control dogs were included in the study. Dogs with MMVD had significantly higher vitamin E, ACL, MDA, and cholesterol concentrations and significantly higher LS-VitE values than control dogs. No significant correlations between MDA and antioxidant parameters were determined in either group. In conclusion, oxidative damage to lipids is already present and the antioxidant status is altered but not depleted in dogs with MMVD ACVIM B1. The antioxidant response to increased oxidative damage consists mainly of the activation of fat-soluble antioxidants. Further research is needed to evaluate the efficacy and targets of early antioxidant supplementation to prevent or ameliorate oxidative stress and mitigate disease progression in dogs with early-stage MMVD.

Keywords: antioxidant status; dog; lipid peroxidation; myxomatous mitral valve degeneration; oxidative stress.