Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential

Yang Deng; Tong Mou; Junhuan Wang; Jing Su; Yanchun Yan; Yu-Qin Zhang

doi:10.3389/fmicb.2023.1225746

Characterization of three rapidly growing novel Mycobacterium species with significant polycyclic aromatic hydrocarbon bioremediation potential

Front Microbiol. 2023 Sep 8:14:1225746. doi: 10.3389/fmicb.2023.1225746. eCollection 2023.

Authors

Yang Deng^{1

2}, Tong Mou^{1

2}, Junhuan Wang³, Jing Su¹, Yanchun Yan³, Yu-Qin Zhang^{1

2}

Affiliations

¹ Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
² State Key Laboratory of Dao-di Herbs, Beijing, China.
³ Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China.

Abstract

Mycobacterium species exhibit high bioremediation potential for the degradation of polycyclic aromatic hydrocarbons (PAHs) that are significant environmental pollutants. In this study, three Gram-positive, rapidly growing strains (YC-RL4^T, MB418^T, and HX176^T) were isolated from petroleum-contaminated soils and were classified as Mycobacterium within the family Mycobacteriaceae. Genomic average nucleotide identity (ANI; < 95%) and digital DNA-DNA hybridization (dDDH; < 70%) values relative to other Mycobacterium spp. indicated that the strains represented novel species. The morphological, physiological, and chemotaxonomic characteristics of the isolates also supported their affiliation with Mycobacterium and their delineation as novel species. The strains were identified as Mycobacterium adipatum sp. nov. (type strain YC-RL4^T = CPCC 205684^T = CGMCC 1.62027^T), Mycobacterium deserti sp. nov. (type strain MB418^T = CPCC 205710^T = KCTC 49782^T), and Mycobacterium hippophais sp. nov. (type strain HX176^T = CPCC 205372^T = KCTC 49413^T). Genes encoding enzymes involved in PAH degradation and metal resistance were present in the genomes of all three strains. Specifically, genes encoding alpha subunits of aromatic ring-hydroxylating dioxygenases were encoded by the genomes. The genes were also identified as core genes in a pangenomic analysis of the three strains along with 70 phylogenetically related mycobacterial strains that were previously classified as Mycolicibacterium. Notably, strain YC-RL4^T could not only utilize phthalates as their sole carbon source for growth, but also convert di-(2-ethylhexyl) phthalate into phthalic acid. These results indicated that strains YC-RL4^T, MB418^T, and HX176^T were important resources with significant bioremediation potential in soils contaminated by PAHs and heavy metals.

Keywords: Mycobacterium adipatum; Mycobacterium deserti; Mycobacterium hippophais; bioremediation potential; genome.