A method for in vivo evaluation of α-glucosidase inhibition using Drosophila

Nattapong Wongchum; Alongklod Tanomtong; Somchai Pinlaor; Chatmongkon Suwannapoom; Ananya Dechakhamphu

doi:10.1016/j.mex.2023.102373

A method for in vivo evaluation of α-glucosidase inhibition using Drosophila

MethodsX. 2023 Sep 9:11:102373. doi: 10.1016/j.mex.2023.102373. eCollection 2023 Dec.

Authors

Nattapong Wongchum¹, Alongklod Tanomtong², Somchai Pinlaor³, Chatmongkon Suwannapoom⁴, Ananya Dechakhamphu^{5

6}

Affiliations

¹ Biology Program, Faculty of Science, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000 Thailand.
² Biology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
³ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
⁴ Department of Fishery, School of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand.
⁵ Aesthetic Sciences and Health Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000 Thailand.
⁶ Thai Traditional Medicine Program, Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubonratchathani 34000 Thailand.

Abstract

The development of α-glucosidase inhibitors is essential for the prevention of type II diabetes. Previous research has investigated in vitro inhibition using isolated α-glucosidase, which may not accurately reflect physical processes. The method presented in this study aims to establish a rapid and inexpensive in vivo method to study the inhibition of α-glucosidase activity using Drosophila as a model organism. This method can be used to calculate the IC₅₀ value of compounds of interest for inhibition of α-glucosidase activity. The method established in this study can be used for in vivo screening of anti-diabetic compounds. •A rapid and inexpensive in vivo method to study the inhibition of α-glucosidase activity.•This method can be used to calculate the IC₅₀ value of compounds of interest for inhibition of α-glucosidase activity.•This is a useful method for in vivo screening of anti-diabetic compounds.

Keywords: Animal model; Anti-diabetic assay; Anti-glucosidase activity; Evaluating the inhibition of active compound on α-glucosidase activity using Drosophila as a model; In vivo screening; Inhibition assay.