Long and Short-terms Effects of Ablative Fractional Laser Therapy on Human Skin: A Network Analysis

Zahra Razzaghi; Babak Arjmand; Mostafa Rezaei Tavirani; Maryam Hamzeloo-Moghadam; Mohammad Rostami Nejad; Reza Mohamoud Robati

doi:10.34172/jlms.2023.27

Long and Short-terms Effects of Ablative Fractional Laser Therapy on Human Skin: A Network Analysis

J Lasers Med Sci. 2023 Aug 27:14:e27. doi: 10.34172/jlms.2023.27. eCollection 2023.

Authors

Zahra Razzaghi¹, Babak Arjmand^{2

3}, Mostafa Rezaei Tavirani⁴, Maryam Hamzeloo-Moghadam⁵, Mohammad Rostami Nejad⁶, Reza Mohamoud Robati⁷

Affiliations

¹ Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
³ Iranian Cancer Control Center (MACSA), Tehran, Iran.
⁴ Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁵ Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine Shahid, Beheshti University of Medical Sciences, Tehran, Iran.
⁶ Celiac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
⁷ Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Time-dependent effects of laser radiation have been investigated by researchers. An understanding of the molecular mechanism of the time course effect of the laser needs molecular assessment and function evaluation of the related genes. In the present study, the importance of repetition of treatment after 4 weeks and gene expression alteration after 7 days of laser radiation versus one day on the human skin was evaluated via protein-protein interaction (PPI) network analysis and gene ontology enrichment. Methods: The differentially expressed genes (DEGs) were extracted from Gene Expression Omnibus (GEO) and assessed via PPI network analysis. The critical DEGs were enriched via gene ontology. The related biological processes and biochemical pathways were retrieved from "GO-Biological process" and "Kyoto Encyclopedia of Genes and Genomes" (KEGG) respectively. Results: The repetition of laser therapy after 4 weeks of the first treatment did not have a significant effect on treatment efficacy. Sixty-three significant DEGs and six classes of biological terms discriminated the samples seven days after the treatment from individuals one day after the treatment. The studied DEGs were organized into two clusters with certain functions. Conclusion: Based on the findings after laser therapy, several days are required to complete the critical processes such as DNA biosynthesis and skin cornification.

Keywords: Gene expression change; Gene ontology; Human; Laser radiation; Time of radiation.