Development of a rapid and sensitive fluorescent probe for high-throughput detecting SO2 in food samples

Ziqiang Wang; Nanxin Li; Zhiyuan Xie; Tingting Pan; Chi Zhang; Jianxiang Liao; Yanqing Tian

doi:10.1016/j.foodchem.2023.137506

Development of a rapid and sensitive fluorescent probe for high-throughput detecting SO₂ in food samples

Food Chem. 2024 Feb 15:434:137506. doi: 10.1016/j.foodchem.2023.137506. Epub 2023 Sep 18.

Authors

Ziqiang Wang¹, Nanxin Li², Zhiyuan Xie¹, Tingting Pan³, Chi Zhang⁴, Jianxiang Liao⁵, Yanqing Tian⁶

Affiliations

¹ School of Medicine, Southern University of Science and Technology, No 1088 Xueyuan Blvd, Xili, Nanshan District, Shenzhen, Guangdong 518055, China.
² Guangzhou Quality Supervision and Testing Institute, Guangzhou 51000, China.
³ Department of Pediatric Neurology, Shenzhen Children's Hospital, 7019 Yitian Road, Shenzhen 518038, China.
⁴ Department of Laboratory Medicine, Shenzhen University General Hospital, Shenzhen 518071, China.
⁵ Department of Pediatric Neurology, Shenzhen Children's Hospital, 7019 Yitian Road, Shenzhen 518038, China. Electronic address: liaojianxiang@vip.sina.com.
⁶ Department of Materials Science and Engineering, Southern University of Science and Technology, No 1088 Xueyuan Blvd, Xili, Nanshan District, Shenzhen, Guangdong 518055, China. Electronic address: tianyq@sustech.edu.cn.

PMID: 37742548
DOI: 10.1016/j.foodchem.2023.137506

Abstract

Sulfur dioxide (SO₂), widely used as an antioxidant and preservative in food production, has been associated with detrimental cardiovascular and neurological effects when consumed excessively. This highlights the pressing need to develop a fast and sensitive probe capable of high-throughput screening for the quantitative determination of SO₂ in food. Herein, we synthesized a new fluorescent probe, namely B3, specifically designed for high-throughput detection of SO₂ in food. The vinyl chloride aldehyde within the B3 structure engages in a nucleophilic addition reaction with SO₂, contributing to B3's exceptional selectivity for SO₂, and fast response time within 9 s. Furthermore, by integrating B3 with a microplate reader, we effectively achieved high-throughput detection of SO₂ concentration up to 45 μM within a pH range of 5.5 to 8.0 in real food samples. This accomplishment serves as a significant contribution to ensuring consumer safety and facilitating health assessments.

Keywords: Fluorescent probe; High-throughput detection; Real food samples; Sulfur dioxide.