Short communication: Quantifying postruminal starch fermentation in early-lactation Holstein-Friesian cows

S van Gastelen; J Dijkstra; W J J Gerrits; M S Gilbert; A Bannink

doi:10.1016/j.animal.2023.100974

Short communication: Quantifying postruminal starch fermentation in early-lactation Holstein-Friesian cows

Animal. 2023 Oct;17(10):100974. doi: 10.1016/j.animal.2023.100974. Epub 2023 Aug 29.

Authors

S van Gastelen¹, J Dijkstra², W J J Gerrits², M S Gilbert², A Bannink³

Affiliations

¹ Wageningen Livestock Research, Wageningen University & Research, PO Box 338, 6700 AH Wageningen, the Netherlands. Electronic address: sanne.vangastelen@wur.nl.
² Animal Nutrition Group, Wageningen University & Research, PO Box 338, 6700 AH Wageningen, the Netherlands.
³ Wageningen Livestock Research, Wageningen University & Research, PO Box 338, 6700 AH Wageningen, the Netherlands.

PMID: 37742498
DOI: 10.1016/j.animal.2023.100974

Abstract

It has previously been shown that fermentation may contribute substantially to small intestinal carbohydrate disappearance. The fact that the energetic efficiency of starch fermentation is considerably less than that of enzymatic digestion of starch, makes it of nutritional importance to quantify the level of postruminal starch fermentation for dairy cows. Hence, we subjected six rumen-fistulated Holstein-Friesian dairy cows (48 ± 17 days in milk) to 5 d of continuous abomasal infusions of 0.0, 2.5, and 5.0 mol NH₄Cl/d, with and without 3 kg ground maize/d, followed by 2 d of rest in a 6 × 6 Latin square design. A total mixed ration (TMR) consisting of (DM basis) 70% grass silage and 30% concentrate was fed at 95% of ad libitum intake. Separation of postruminal starch disappearance into enzymatically digested starch and fermented starch was based on the measurement of natural ¹³C enrichment of the TMR, abomasally infused ground maize, and resulting ¹³C enrichment of faeces. Within each cow, 0.0, 2.5, and 5.0 mol NH₄Cl/d without ground maize served as control for the same levels of NH₄Cl with 3 kg ground maize/d. Abomasal infusion of ground maize was associated with increased total DM and starch intake, faecal starch excretion, and digestibility of starch, and with decreased digestibility of DM and N. The increased faecal volatile fatty acid (VFA) output and ¹³C enrichment of the individual VFA indicate increased starch fermentation with abomasally infused ground maize. On average, 1 311 g starch/d was postruminally fermented, representing 60.8% of total starch intake. Overall, postruminal starch fermentation of early-lactation dairy cows abomasally infused with 3 kg ground maize/d is considerable and may result in substantial amounts of VFA rather than glucose production.

Keywords: (13)C enrichment; Dairy cattle; Digestion; Fermentation; Maize starch.