Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry

Mathieu Melczer; Javier Jiménez Lamana; Ana Justo-Vega; Ogier Hanser; Sophie Ndaw; Ryszard Lobinski

doi:10.1016/j.talanta.2023.125226

Multielement analysis of single red blood cells by single cell - inductively coupled plasma tandem mass spectrometry

Talanta. 2024 Jan 15:267:125226. doi: 10.1016/j.talanta.2023.125226. Epub 2023 Sep 20.

Authors

Mathieu Melczer¹, Javier Jiménez Lamana², Ana Justo-Vega³, Ogier Hanser⁴, Sophie Ndaw⁴, Ryszard Lobinski⁵

Affiliations

¹ French Research and Safety Institute for the Prevention of Occupational Accidents and Diseases (INRS), Toxicology and Biomonitoring Division, 1 Rue Morvan, F-54519, Vandoeuvre les Nancy, France. Electronic address: mathieu.melczer@inrs.fr.
² Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, Institute of Analytical and Physical Chemistry for Environment and Materials (IPREM-UMR5254), 64053, Pau, France.
³ Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, Institute of Analytical and Physical Chemistry for Environment and Materials (IPREM-UMR5254), 64053, Pau, France; Group of Trace Elements, Spectroscopy and Speciation (GETEE), Materials Institute (iMATUS), Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Chemistry, University of Santiago de Compostela, Avda das Ciencias, s/n, 15782, Santiago de Compostela, Spain.
⁴ French Research and Safety Institute for the Prevention of Occupational Accidents and Diseases (INRS), Toxicology and Biomonitoring Division, 1 Rue Morvan, F-54519, Vandoeuvre les Nancy, France.
⁵ Universite de Pau et des Pays de l'Adour, E2S UPPA, CNRS, Institute of Analytical and Physical Chemistry for Environment and Materials (IPREM-UMR5254), 64053, Pau, France; Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, ul. Noakowskiego 3, 00-664, Warswawa, Poland.

PMID: 37742394
DOI: 10.1016/j.talanta.2023.125226

Abstract

A method for the analysis of essential metals (Fe, Cu, Mg, and Zn) and non-metals (P, S) in single red blood cells was developed by single cell (SC)-ICP-MS. The use of a triple quadrupole configuration (MS/MS) enabled an effective elimination of polyatomic interferences, which affect the accuracy of ICP-MS analysis using a single quadrupole mass analyzer. Fixation with glutaraldehyde for at least 90 days was developed to improve the quantification of elements in a single red blood cell. The experimental conditions were optimized while special attention was paid to the residence time of analytes in the plasma. Addition of a surfactant (0.05% (v/v) Tween80®) improved quantification of elements in fixed red blood cells. The detection limits obtained by SC-ICP-MS/MS were lower than for ICP-MS, especially for S and P (3 fg and 1.7 fg. cell^-1 instead of 163 and 6.3 fg. cell^-1, respectively).

Keywords: Erythrocytes; Fixation; ICP-MS/MS; Red blood cells; SC-ICP-MS; Single cell.

MeSH terms

Erythrocytes / chemistry
Metals* / analysis
Spectrum Analysis
Tandem Mass Spectrometry*

Substances

Metals