Protective mechanism of milk fat globule membrane proteins on Lactobacillus acidophilus CICC 6074 under acid stress based on proteomic analysis

Tao Zhang; Yujie Yang; Xiaoqun Zeng; Zhen Wu; Daodong Pan; Haibo Luo; Mingxuan Tao; Yuxing Guo

doi:10.1016/j.foodchem.2023.137297

Protective mechanism of milk fat globule membrane proteins on Lactobacillus acidophilus CICC 6074 under acid stress based on proteomic analysis

Food Chem. 2024 Feb 15:434:137297. doi: 10.1016/j.foodchem.2023.137297. Epub 2023 Aug 28.

Authors

Tao Zhang¹, Yujie Yang², Xiaoqun Zeng³, Zhen Wu³, Daodong Pan³, Haibo Luo², Mingxuan Tao², Yuxing Guo⁴

Affiliations

¹ Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, Jiangsu, PR China; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, Zhejiang, PR China.
² Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, Jiangsu, PR China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, Zhejiang, PR China.
⁴ Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, Jiangsu, PR China. Electronic address: guoyuxing1981@163.com.

PMID: 37741242
DOI: 10.1016/j.foodchem.2023.137297

Abstract

The prerequisite for lactic acid bacteria to perform their probiotic function is that they could survive the acid-stressed environment of production and application. In this experiment, the protective mechanism of milk fat globule membrane (MFGM) proteins on lactic acid bacteria under acid stress was investigated. Scanning electron microscopy and fluorescence probe were used to analyze the condition of the acid-treated bacteria, which showed that MFGM proteins could enhance the survival ability of Lactobacillus acidophilus CICC 6074 under acid stress by maintaining cell morphology, elevating intracellular pH and H⁺-ATPase activity. Furthermore, Tandem Mass Tags (TMT) proteomic analysis revealed that MFGM protein could exert protective effects on L. acidophilus CICC 6074 by regulating amino acid metabolism, ATPase activity, peptidoglycan synthesis, gene repair and heritage, etc. The results will provide a new approach for the protection and development of functional lactic acid bacteria.

Keywords: Acid tolerance; Lactobacillus acidophilus CICC 6074; MFGM proteins; Proteome.