Chemical characterization, anti-struvite crystal, anti-inflammatory, analgesic, and antidepressant activities of Papaver rhoeas L. root and leaf extracts

Abstract

Ethnopharmacological relevance: Papaver rhoeas L. (P. rhoeas) is used to treat a wide range of pathologies. But there is no data on the anti-struvite, anti-inflammatory, analgesic, and anti-depressant effects of root and leaf extracts from this medicinal plant.

Aim of the study: This study aimed to chemically characterize and determine the antidepressant, analgesic, anti-inflammatory, and anti-struvite activities of the root extract (RE) and leaf extract (LE) of P. rhoeas, a wild plant.

Materials and methods: The extraction of secondary metabolites and phytochemical screening were carried out using standard methods. The anti-struvite effect of our samples was assessed by Fourier transform infrared spectroscopy (FT-IR) and microscopic observation. For the anti-inflammatory impact, we used the carrageenan-induced paw edema technique in rats. To assess the analgesic effect of the extracts, we recorded the resulting abdominal contraction after intraperitoneal (IP) acetic acid injection. Finally, we assessed the antidepressant effect by conducting the Forced Swimming Test (FST).

Results: Phytochemical screening results revealed the presence of phenols, flavonoids, saponins, tannins, coumarins, terpenoids, and alkaloids. Extraction results confirmed that our extracts were rich in secondary metabolites. Both P. rhoeas extracts limited struvite crystal development by reducing the size and number of crystals, as indicated by FT-IR analysis and microscopic observation. Additionally, the anti-struvite effects of the LE sample were greater than those of the RE sample. The results of the anti-inflammatory capacity of our extracts demonstrate the maximum inhibition of carrageenan-induced edema following 6 h (T₆) of injection, ranging from 72.73 ± 7.7% for LE (400 mg/kg) to 95.45 ± 6.42% for the RE sample (400 mg/kg). On the other hand, the results for analgesic activity showed that the number of abdominal contractions in rats treated with RE and LE ranged from 11 ± 1.73 for RE (400 mg/kg) to 18 ± 1.50 for LE (200 mg/kg), while that of aspirin was 10.33 ± 0.57. Furthermore, the FST results show that the immobility time in rats treated with RE and LE varied from 91.98 ± 4.24 s for LE-treated rats (300 mg/kg) to 123.54 ± 7.83 s for RE-treated rats (150 mg/kg).

Conclusion: Based on these results, the roots and leaves of the studied plant can be considered a significant source of biologically active molecules for use in the pharmaceutical industry.

Keywords: Analgesic; Anti-inflammatory; Anti-struvite crystal; Antidepressant; Papaver rhoeas L..