Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA)

Changqi Liu; Mengna Su; Valerie D Champ; Sahil Gupta

doi:10.1007/978-1-0716-3453-0_19

Validation Procedures for Quantification of Food Allergens by Enzyme-Linked Immunosorbent Assay (ELISA)

Methods Mol Biol. 2024:2717:285-304. doi: 10.1007/978-1-0716-3453-0_19.

Authors

Changqi Liu¹, Mengna Su², Valerie D Champ³, Sahil Gupta⁴

Affiliations

¹ School of Exercise and Nutritional Sciences, San Diego State University, San Diego, CA, USA. changqi.liu@sdsu.edu.
² Fujian Hwa Nan Women's College, Fuzhou, Fujian, China.
³ PerkinElmer Inc., Austin, TX, USA.
⁴ General Mills, Inc., Minneapolis, MN, USA.

PMID: 37737992
DOI: 10.1007/978-1-0716-3453-0_19

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a widely used analytical technique for food allergen detection and quantification. Validating ELISA protocols is important for both assay developers and end users as it ensures method reliability. This chapter describes the protocols for validating the sensitivity, specificity, precision, accuracy, robustness, and ruggedness of an ELISA. Example procedures are also provided for sample preparation, allergen extraction, and ELISA operation.

Keywords: Allergen detection; Assay validation; ELISA; Food allergy; Immunoassay.

MeSH terms

Biological Assay*
Enzyme-Linked Immunosorbent Assay
Reproducibility of Results
Research Design*
Specimen Handling