Occurrence of Diarrheagenic Pathogens and Their Coinfection Profiles in Diarrheic Under Five Children and Tracked Human Contacts in Urban and Rural Settings of Eastern Ethiopia

Dinaol Belina; Tesfaye Gobena; Ameha Kebede; Meseret Chimdessa; Yonas Hailu; Tine Hald

doi:10.1177/11786361231196527

Occurrence of Diarrheagenic Pathogens and Their Coinfection Profiles in Diarrheic Under Five Children and Tracked Human Contacts in Urban and Rural Settings of Eastern Ethiopia

Microbiol Insights. 2023 Sep 19:16:11786361231196527. doi: 10.1177/11786361231196527. eCollection 2023.

Authors

Dinaol Belina^{1

2}, Tesfaye Gobena³, Ameha Kebede², Meseret Chimdessa², Yonas Hailu⁴, Tine Hald⁵

Affiliations

¹ College of Veterinary Medicine, Haramaya University, Dire Dawa, Ethiopia.
² School of Biological Sciences and Biotechnology, Haramaya University, Dire Dawa, Ethiopia.
³ College of Health and Medical Sciences, Haramaya University, Harar, Ethiopia.
⁴ Teagasc Food Research Centre, Moorepark, Fermoy, County Cork, Ireland.
⁵ National Food Institute, Technical University of Denmark, Lyngby, Denmark.

Abstract

Diarrheagenic Escherichia coli, Campylobacter, Nontyphoidal Salmonella, and Shigella are common cause of childhood diarrhea in countries like Ethiopia, but data on their sources and coinfection profiles is limited. A cross sectional study was conducted from November 2021 to January 2023 to determine the prevalence, coinfection, and monthly occurrence rates of major diarrheagenic bacteria in diarrheic under five children and asymptomatic contacts at urban and rural settings in Ethiopia. A total of 345 stool samples were collected from; 262 diarrheic children visiting Hiwot Fana Hospital, Kersa, and Adelle Health Centers; and 83 caretakers and siblings through case based contact tracing. Samples were analyzed using standard laboratory procedures and the overall prevalence of enteric pathogens was 26.96%, with the highest isolation rate during the winter and peaks of 73.91% in February. The occurrence of the pathogens in children and tracked contacts was 27.86 and 24.09%, respectively. In our study, 8.53% coinfection and 23.66% single pathogen infection was recorded in diarrheic children. The study also showed 4.51 and 3.88% of diarrhea in children from urban and rural had attributed to bacterial coinfection, respectively. The most prevalent pathogen in diarrheic children was Diarrheagenic E. coli (10.31%), and followed by Campylobacter. On the other hand, Diarrheagenic E. coli was the second dominant bacteria following Shigella in the traced contacts, with prevalence of 8.43% and 9.64%, respectively. Based on the study site, the prevalence of Diarrheagenic E. coli and Nontyphoidal Salmonella was higher in children from urban than those from rural. However, the occurrence of each pathogen had no significant differences (P > .05) between settings. The high pathogens occurrence rate in the current study indicates the need for strong control strategies and better child carrying and treatment of diarrheal diseases at both urban and rural settings. Further studies on possible sources and factors attributing to the occurrence of enteric pathogens in children are also recommended.

Keywords: Diarrhea; Ethiopia; enteric bacteria; human contact; under five children.