Exercise evaluation with metabolic and ventilatory responses and blood lactate concentration in mice

Akira Yoshikawa; Makito Iizuka; Mitsuko Kanamaru; Shotaro Kamijo; Hirokazu Ohtaki; Masahiko Izumizaki

doi:10.1016/j.resp.2023.104163

Exercise evaluation with metabolic and ventilatory responses and blood lactate concentration in mice

Respir Physiol Neurobiol. 2023 Dec:318:104163. doi: 10.1016/j.resp.2023.104163. Epub 2023 Sep 19.

Authors

Akira Yoshikawa¹, Makito Iizuka², Mitsuko Kanamaru³, Shotaro Kamijo⁴, Hirokazu Ohtaki⁵, Masahiko Izumizaki²

Affiliations

¹ Department of Physiology, Showa University School of Medicine, Tokyo, Japan; Division of Health Science Education, Showa University School of Nursing and Rehabilitation Sciences, Yokohama, Japan. Electronic address: yoshi-aki@med.showa-u.ac.jp.
² Department of Physiology, Showa University School of Medicine, Tokyo, Japan.
³ Department of Physiology, Showa University School of Medicine, Tokyo, Japan; Faculty of Arts and Sciences at Fujiyoshida, Showa University, Yamanashi, Japan.
⁴ Department of Physiology, Showa University School of Medicine, Tokyo, Japan; Department of Physiology, Showa University School of Pharmacy, Tokyo, Japan.
⁵ Department of Functional Neurobiology, Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Hachioji, Japan; Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.

PMID: 37734454
DOI: 10.1016/j.resp.2023.104163

Abstract

This study aimed to clarify the differential exercise capacity between 2-month-old and 10-month-old mice using an incremental running test. Metabolic and ventilatory responses and blood lactate concentration were measured to evaluate exercise capacity. We examined whether incremental running test results reflected metabolic and ventilatory responses and blood lactate concentration observed during the steady-state running test. Metabolic response significantly declined with age, whereas ventilatory response was similar between the groups. A low-intensity/moderate exercise load of 10/min in an incremental running test was performed on both mice for 30 min. They showed a characteristic pattern in ventilatory response in 10-month mice. The results of incremental running tests didn't necessarily reflect the steady-state metabolic and ventilatory responses because some parameters showed an approximation and others did not in incremental and steady-state tests, which changed with age. Our study suggests metabolic and ventilatory responses depending on age and provides basic knowledge regarding the objective and quantitative assessment of treadmill running in an animal model.

Keywords: Age; Evaluation; Exercise; Lactate; Pulmonary gas exchange; Ventilatory responses.