Derivative of cinnamic acid inhibits T3SS of Xanthomonas oryzae pv. oryzae through the HrpG-HrpX regulatory cascade

Yu Shi; Lan-Tu Xiong; Hui Li; Wen-Long Li; Dylan O'Neill Rothenberg; Li-Sheng Liao; Xin Deng; Gao-Peng Song; Zi-Ning Cui

doi:10.1016/j.bioorg.2023.106871

Derivative of cinnamic acid inhibits T3SS of Xanthomonas oryzae pv. oryzae through the HrpG-HrpX regulatory cascade

Bioorg Chem. 2023 Dec:141:106871. doi: 10.1016/j.bioorg.2023.106871. Epub 2023 Sep 18.

Authors

Yu Shi¹, Lan-Tu Xiong², Hui Li³, Wen-Long Li², Dylan O'Neill Rothenberg⁴, Li-Sheng Liao², Xin Deng⁵, Gao-Peng Song⁶, Zi-Ning Cui⁷

Affiliations

¹ National Key Laboratory of Green Pesticide, Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China; Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
² National Key Laboratory of Green Pesticide, Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
³ College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
⁴ College of Horticulture Science, South China Agricultural University, Guangzhou 510642, China.
⁵ Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR 999077, China; Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China.
⁶ College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China. Electronic address: songgp1021@scau.edu.cn.
⁷ National Key Laboratory of Green Pesticide, Integrative Microbiology Research Centre, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China. Electronic address: ziningcui@scau.edu.cn.

PMID: 37734193
DOI: 10.1016/j.bioorg.2023.106871

Abstract

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) has a significant impact on rice yield and quality worldwide. Traditionally, bactericide application has been commonly used to control this devastating disease. However, the overuse of fungicides has led to a number of problems such as the development of resistance and environmental pollution. Therefore, the development of new methods and approaches for disease control are still urgent. In this paper, a series of cinnamic acid derivatives were designed and synthesized, and three novel T3SS inhibitors A10, A12 and A20 were discovered. Novel T3SS inhibitors A10, A12 and A20 significantly inhibited the hpa1 promoter activity without affecting Xoo growth. Further studies revealed that the title compounds A10, A12 and A20 significantly impaired hypersensitivity in non-host plant tobacco leaves, while applications on rice significantly reduced symptoms of bacterial leaf blight. RT-PCR showed that compound A20 inhibited the expression of T3SS-related genes. In summary, this work exemplifies the potential of the title compound as an inhibitor of T3SS and its efficacy in the control of bacterial leaf blight.

Keywords: Cinnamic acid; Derivative; Inhibitor; T3SS; Xanthomonas oryzae pv. oryzae (Xoo).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cinnamates / metabolism
Cinnamates / pharmacology
Oryza* / metabolism
Type III Secretion Systems / genetics
Type III Secretion Systems / metabolism
Xanthomonas* / metabolism

Substances

cinnamic acid
antineoplaston A10
Type III Secretion Systems
Cinnamates

Supplementary concepts

Xanthomonas oryzae