Nano selenium-doped TiO2 nanotube arrays on orthopedic implants for suppressing osteosarcoma growth

Xiaodong Hu; Chunhai Ke; Jiaqi Zhong; Yujiong Chen; Jieyang Dong; Mingming Hao; Qi Chen; Jiahua Ni; Zhaoxiang Peng

doi:10.3389/fbioe.2023.1252816

Nano selenium-doped TiO₂ nanotube arrays on orthopedic implants for suppressing osteosarcoma growth

Front Bioeng Biotechnol. 2023 Sep 5:11:1252816. doi: 10.3389/fbioe.2023.1252816. eCollection 2023.

Authors

Xiaodong Hu^#¹, Chunhai Ke^#¹, Jiaqi Zhong¹, Yujiong Chen¹, Jieyang Dong¹, Mingming Hao^{1

2}, Qi Chen³, Jiahua Ni³, Zhaoxiang Peng¹

Affiliations

¹ Affiliated Li Huili Hospital, Ningbo University, Ningbo, China.
² Ningbo Institute of Innovation for Combined Medicine and Engineering (NIIME), Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, China.
³ Ningbo Regen Biotech, Co, Ltd, Ningbo, Zhejiang, China.

^# Contributed equally.

Abstract

Osteosarcoma, the most common primary malignant bone tumor, is characterized by malignant cells producing osteoid or immature bone tissue. Most osteosarcoma patients require reconstructive surgery to restore the functional and structural integrity of the injured bone. Metal orthopedic implants are commonly used to restore the limb integrity in postoperative patients. However, conventional metal implants with a bioinert surface cannot inhibit the growth of any remaining cancer cells, resulting in a higher risk of cancer recurrence. Herein, we fabricate a selenium-doped TiO₂ nanotube array (Se-doped TNA) film to modify the surface of medical pure titanium substrate, and evaluate the anti-tumor effect and biocompatibility of Se-doped TNA film. Moreover, we further explore the anti-tumor potential mechanism of Se-doped TNA film by studying the behaviors of human osteosarcoma cells in vitro. We provide a new pathway for achieving the anti-tumor function of orthopedic implants while keeping the biocompatibility, aiming to suppress the recurrence of osteosarcoma.

Keywords: TiO2 nanotube arrays; anti-tumor effect; nano selenium; orthopedic titanium implant; osteosarcoma.

Grants and funding

The authors are grateful for support from Zhejiang Provincial Natural Science Foundation of China under Grant (No. LGF21H060003), the “Science and Technology Innovation 2025” Major Special Project of Ningbo (No. 2019B10064), the foundation project for medical science and technology of Zhejiang Province (No. 2022KY1083), Ningbo Yongjiang Talent Introduction Programme (No. 2021A-007-G), and the Natural Science Foundation of Ningbo Municipality (No. 202003N4067).