Disposable nanosensor for the electrochemical determination of the interaction between DNA, and a mycotoxin, patulin

Fatma Akpınar; Şerife Gökçe Çalışkan; Mihrican Muti

doi:10.1016/j.jpba.2023.115713

Disposable nanosensor for the electrochemical determination of the interaction between DNA, and a mycotoxin, patulin

J Pharm Biomed Anal. 2023 Nov 30:236:115713. doi: 10.1016/j.jpba.2023.115713. Epub 2023 Sep 7.

Authors

Fatma Akpınar¹, Şerife Gökçe Çalışkan², Mihrican Muti³

Affiliations

¹ Aydın Adnan Menderes University, Faculty of Sciences, Department of Chemistry, 09100 Aydın, Turkey.
² Aydın Adnan Menderes University, Faculty of Sciences, Department of Physics, 09100 Aydın, Turkey.
³ Aydın Adnan Menderes University, Faculty of Sciences, Department of Chemistry, 09100 Aydın, Turkey. Electronic address: mihricanmuti@adu.edu.tr.

PMID: 37729744
DOI: 10.1016/j.jpba.2023.115713

Abstract

Silicon dioxide nanoparticles were synthesized and disposable screen-printed electrodes were modified with these nanoparticles to electrochemically detect the interaction between DNA and patulin, a mycotoxin. Firstly, the synthesized silicon dioxide nanoparticles were chemically characterized by X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FT-IR). Microscopic characterization of the nanoparticles was performed by Transmission Electron Microscopy (TEM) and Energy-dispersive X-ray spectroscopy (EDX). The surface of the silicon dioxide nanoparticle-modified screen-printed electrode was characterized by Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). SiNP modification resulted in a 2-fold increase in surface area and a 2.3-fold enhancement in the signal. The detection limit (LOD) for the electrochemical patulin determination was calculated as 1.15 µg/mL, and the linear concentration range was found to be 3.2-20 µg/mL. The mode of interaction between patulin and dsDNA was determined through a molecular docking study. After the interaction between patulin and dsDNA, approximately 86 % and 23 % decreases were observed in patulin and guanine oxidation signals, respectively. The S % value for patulin was calculated by utilizing the decrease in the guanine signal after the interaction.

Keywords: Mycotoxin; Nanosensor; Patulin; Screen-printed electrode; Silicon dioxide.