Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18

Shiying Zhou; Shuyu Zhu; Zhen Huang; Jian Chen; Jiawei Li; Mei Yang; Liang Jin; Danqun Huo; Changjun Hou

doi:10.1039/d3cc04223e

Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18

Chem Commun (Camb). 2023 Oct 5;59(80):11987-11990. doi: 10.1039/d3cc04223e.

Authors

Shiying Zhou¹, Shuyu Zhu¹, Zhen Huang², Jian Chen³, Jiawei Li³, Mei Yang¹, Liang Jin¹, Danqun Huo¹, Changjun Hou^{1

4}

Affiliations

¹ Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China.
² Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, SeNA Research Institute and Szostak-CDHT Large Nucleic Acids Institute, Chengdu, 610000, PR China.
³ Chongqing University Three Gorges Hospital, Chongqing, 404000, PR China.
⁴ Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, PR China. houcj@cqu.edu.cn.

PMID: 37727048
DOI: 10.1039/d3cc04223e

Abstract

Simultaneous detection of multiple targets can provide important data support for clinical diagnosis and treatment. Here, we report a facile isothermal assay based on target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a (TM-RCT/Cas12a-Cas13a). Through facile one-step amplification (TM-RCT), two target DNAs are converted to RNA amplified products. The simultaneous detection of HPV16 and HPV18 is then achieved by combining two CRISPR/Cas systems. This system shows excellent sensing performance and provides a universal method for simultaneous detection.