Molecular cytogenetic analysis of the olive flounder embryonic cell line FGBC8 and its applicability to biotechnology

A-Young Jeon; Ja Young Cho; Jungwook Park; Woo-Jin Kim; Young-Ok Kim; Hee Jeong Kong; Ju-Won Kim

doi:10.1016/j.fsi.2023.109077

Molecular cytogenetic analysis of the olive flounder embryonic cell line FGBC8 and its applicability to biotechnology

Fish Shellfish Immunol. 2023 Nov:142:109077. doi: 10.1016/j.fsi.2023.109077. Epub 2023 Sep 17.

Authors

A-Young Jeon¹, Ja Young Cho¹, Jungwook Park¹, Woo-Jin Kim¹, Young-Ok Kim¹, Hee Jeong Kong¹, Ju-Won Kim²

Affiliations

¹ Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
² Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea. Electronic address: ogamzar@korea.kr.

PMID: 37726081
DOI: 10.1016/j.fsi.2023.109077

Abstract

We explored the biotechnological applicability of a previously established olive flounder (Paralichthys olivaceus) embryonic cell line (FGBC8). FGBC8 was transfected with pEGFP-c1 and pluripotency-related genes, then infected with viral hemorrhagic septicemia virus (VHSV), and the expression of immune-related genes was observed through quantitative real-time polymerase chain reaction. Transfected cells showed strong green fluorescence 48 h after transfection, and pluripotency-related genes were successfully transfected. In addition, FGBC8 cells were highly susceptible to VHSV and the expression of immune-related genes was induced during infection. Our results demonstrate that FGBC8 cells are valuable research tools for assessing host-pathogen interactions and biotechnological applications.

Keywords: Embryonic cell line; Immune-related gene expression; Olive flounder; Transfection; VHSV.

MeSH terms

Animals
Cell Line
Cytogenetic Analysis
Fish Diseases*
Flounder* / genetics
Hemorrhagic Septicemia, Viral*
Novirhabdovirus* / genetics