CRISPR/Cas9 mediated genome editing of Caenorhabditis nigoni using the conserved dpy-10 co-conversion marker

MicroPubl Biol. 2023 Aug 30:2023:10.17912/micropub.biology.000937. doi: 10.17912/micropub.biology.000937. eCollection 2023.

Abstract

In this study, we developed an efficient co-conversion marker, using the conserved dpy-10 gene, to facilitate creation and detection of CRISPR/Cas9-mediated targeted genomic changes in an emerging male/female nematode model system, Caenorhabditis nigoni .