Grapevine plantlets respond to different monochromatic lights by tuning photosynthesis and carbon allocation

Menglong Liu; Yan Zhao; Peige Fan; Junhua Kong; Yongjian Wang; Xiaobo Xu; Meilong Xu; Lijun Wang; Shaohua Li; Zhenchang Liang; Wei Duan; Zhanwu Dai

doi:10.1093/hr/uhad160

Grapevine plantlets respond to different monochromatic lights by tuning photosynthesis and carbon allocation

Hortic Res. 2023 Aug 8;10(9):uhad160. doi: 10.1093/hr/uhad160. eCollection 2023 Sep.

Authors

Menglong Liu^{1

2

3}, Yan Zhao^{1

2

3}, Peige Fan^{1

2

3}, Junhua Kong^{1

2}, Yongjian Wang^{1

2}, Xiaobo Xu^{1

2

3}, Meilong Xu⁴, Lijun Wang^{1

2

3}, Shaohua Li^{1

2

3}, Zhenchang Liang^{1

2

3}, Wei Duan^{1

2}, Zhanwu Dai^{1

2

3}

Affiliations

¹ State Key Laboratory of Plant Diversity and Specialty Crops, Beijing Key Laboratory of Grape Sciences and Enology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.
² China National Botanical Garden, Beijing 100093, China.
³ University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Ningxia Horticulture Research Institute, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China.

Abstract

The quality of planting materials is the foundation for productivity, longevity, and berry quality of perennial grapevines with a long lifespan. Manipulating the nursery light spectrum may speed up the production of healthy and high-quality planting vines but the underlying mechanisms remain elusive. Herein, the effects of different monochromatic lights (green, blue, and red) on grapevine growth, leaf photosynthesis, whole-plant carbon allocation, and transcriptome reprograming were investigated with white light as control. Results showed that blue and red lights were favorable for plantlet growth in comparison with white light. Blue light repressed excessive growth, significantly increased the maximum net photosynthetic rate (Pn) of leaves by 39.58% and leaf specific weight by 38.29%. Red light increased the dry weight of the stem by 53.60%, the starch content of the leaf by 53.63%, and the sucrose content of the stem by 230%. Green light reduced all photosynthetic indexes of the grape plantlet. Photosynthetic photon flux density (PPFD)/Ci-Pn curves indicated that blue light affected photosynthetic rate depending on the light intensity and CO₂ concentration. RNA-seq analysis of different organs (leaf, stem, and root) revealed a systematic transcriptome remodeling and VvCOP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1), VvHY5 (ELONGATED HYPOCOTYL5), VvHYH (HY5 HOMOLOG), VvELIP (early light-induced protein) and VvPIF3 (PHYTOCHROME INTERACTING FACTOR 3) may play important roles in this shoot-to-root signaling. Furthermore, the correlation network between differential expression genes and physiological traits indicated that VvpsbS (photosystem II subunit S), Vvpsb28 (photosystem II subunit 28), VvHYH, VvSUS4 (sucrose synthase 4), and VvALDA (fructose-bisphosphate aldolase) were pertinent candidate genes in responses to different light qualities. Our results provide a foundation for optimizing the light recipe of grape plantlets and strengthen the understanding of light signaling and carbon metabolism under different monochromatic lights.