Insight into the mechanism of nutrients removal and response regulation of denitrifying phosphorus removal system under calcium ion stress

Cheng Han; Hongliang Dai; Zechong Guo; Guangcan Zhu; Bing Li; Haq Nawaz Abbasi; Xingang Wang

doi:10.1016/j.biortech.2023.129747

Insight into the mechanism of nutrients removal and response regulation of denitrifying phosphorus removal system under calcium ion stress

Bioresour Technol. 2023 Nov:388:129747. doi: 10.1016/j.biortech.2023.129747. Epub 2023 Sep 17.

Authors

Cheng Han¹, Hongliang Dai², Zechong Guo¹, Guangcan Zhu³, Bing Li⁴, Haq Nawaz Abbasi⁵, Xingang Wang⁶

Affiliations

¹ School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, China.
² School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, China; School of Energy and Environment, Southeast University, Nanjing 210096, China. Electronic address: daihongliang@just.edu.cn.
³ School of Energy and Environment, Southeast University, Nanjing 210096, China.
⁴ Jiangsu Zhongchuang Qingyuan Technology Co., Ltd., Yancheng 224000, China.
⁵ Department of Environmental Science, Federal Urdu University of Arts, Science and Technology, Karachi, Pakistan. Electronic address: hn.abbasi@fuuast.edu.pk.
⁶ School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, China. Electronic address: hofs@just.edu.cn.

PMID: 37717705
DOI: 10.1016/j.biortech.2023.129747

Abstract

The influent quality is an important factor affecting the nutrients removal and operational stability of denitrifying phosphorus removal (DPR) system. This study investigated the effects of calcium ion (Ca²⁺) on the nutrients removal, nitrogen oxide (N₂O) release, microbial community, and quorum sensing in DPR system. Results showed that high accumulation of Ca²⁺ had a significant impact on the carbon footprint of DPR system. Specifically, N₂O release reached 2.11 mg/L under Ca²⁺ of 150 mg/L, which represented 214.93% increase compared to 0 mg/L of Ca²⁺. The DPR system demonstrated its adaptability to elevated Ca²⁺ concentrations by modifying key enzyme activities involved in nitrogen and phosphorus removal, altering the microbial community structure, and adjusting the type and content of signal molecules. These findings hold significant implications for understanding the stress mechanism of Ca²⁺ on DPR system, ultimately aiding in the maintenance and enhancement of stable operational performance in biological wastewater treatment process.

Keywords: Calcium ion; Denitrifying phosphate accumulating organisms; Denitrifying phosphorus removal; Nitrogen oxides; Quorum sensing.