Lanostane triterpenoids with anti-proliferative and PTP1B/α-glucosidase inhibitory activities from the fruiting bodies of Ganoderma calidophilum

Li Yang; Ting-Ting Zhang; Qing-Yun Ma; Qing-Yi Xie; Jiao-Cen Guo; Jia-Ju Lu; Zhi-Fang Yu; Hao-Fu Dai; You-Xing Zhao

doi:10.1016/j.phytochem.2023.113856

Lanostane triterpenoids with anti-proliferative and PTP1B/α-glucosidase inhibitory activities from the fruiting bodies of Ganoderma calidophilum

Phytochemistry. 2023 Nov:215:113856. doi: 10.1016/j.phytochem.2023.113856. Epub 2023 Sep 15.

Authors

Li Yang¹, Ting-Ting Zhang², Qing-Yun Ma¹, Qing-Yi Xie¹, Jiao-Cen Guo¹, Jia-Ju Lu³, Zhi-Fang Yu⁴, Hao-Fu Dai⁵, You-Xing Zhao⁶

Affiliations

¹ Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
² College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
³ Guizhou Institute of Subtropical Crops, Xingyi, Guizhou, 562400, China.
⁴ College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: yuzhifang@njau.edu.cn.
⁵ Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China. Electronic address: daihaofu@itbb.org.cn.
⁶ Key Laboratory of Research and Development of Natural Product from Li Folk Medicine of Hainan Province & National Key Laboratory for Tropical Crop Breeding, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China. Electronic address: zhaoyouxing@itbb.org.cn.

PMID: 37717643
DOI: 10.1016/j.phytochem.2023.113856

Abstract

Twelve previously undescribed and four known lanostane triterpenoids were isolated from the fruiting bodies of Ganoderma calidophilum. The structures of undescribed compounds, ganodecalones H-S (1-12), were elucidated by extensive spectroscopic analysis as well as ECD and NMR calculations. Compound 4 showed significant inhibitory activity against human leukaemia cell line K562, gastric cancer cell line SGC-7901, and cervical cancer cell line HeLa with IC₅₀ values of 13.10 ± 0.19, 17.26 ± 4.75, and 4.36 ± 0.58 μM, respectively. Compound 16 exhibited inhibitory potency against protein tyrosine phosphatase 1B (PTP1B) and α-glucosidase with IC₅₀ values of 30.2 ± 0.13 μM and 120.6 ± 0.14 μM, respectively. The binding sites and interactions of 16 with PTP1B and α-glucosidase were revealed using molecular docking simulations.

Keywords: Anti-proliferative activity; Ganoderma calidophilum; Lanostane triterpenoids; PTP1B; α-glucosidase.

MeSH terms

Fruiting Bodies, Fungal / chemistry
Ganoderma* / chemistry
Humans
Molecular Docking Simulation
Molecular Structure
Steroids / analysis
Triterpenes* / chemistry
alpha-Glucosidases

Substances

Triterpenes
alpha-Glucosidases
Steroids

Supplementary concepts

Ganoderma calidophilum