Unravelling of the genetic diversity, population structure, and phylogenetic relationships of the indigenous horse breeds of Pakistan

Muhammad Sohail Ashraf; Atia Basheer; Farheena Iqbal; Imran Zahoor

doi:10.1016/j.gene.2023.147736

Unravelling of the genetic diversity, population structure, and phylogenetic relationships of the indigenous horse breeds of Pakistan

Gene. 2023 Dec 30:889:147736. doi: 10.1016/j.gene.2023.147736. Epub 2023 Sep 13.

Authors

Muhammad Sohail Ashraf¹, Atia Basheer¹, Farheena Iqbal², Imran Zahoor³

Affiliations

¹ Genetics and Genomics Laboratory, Department of Animal Breeding and Genetics, University of Veterinary and Animal Sciences, Pattoki, Pakistan.
² Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan.
³ Genetics and Genomics Laboratory, Department of Animal Breeding and Genetics, University of Veterinary and Animal Sciences, Pattoki, Pakistan. Electronic address: imran.zahoor@uvas.edu.pk.

PMID: 37714277
DOI: 10.1016/j.gene.2023.147736

Abstract

The current study aimed to evaluate the genetic diversity, level of admixture, and phylogenetic relationship, of the Pakistani horse breeds, along with their morphological characterization. Data for the body measurements showed that Morna horses had the highest values of body height, body length, chest girth, leg length, and head length, whereas the Baluchi horses had the lowest values for these traits. For the genetic diversity 64 animals, 15/breed except for Baluchi(14) and Topras(05), were genotyped by using the 17-plex equine genotyping kit. The AMOVA results showed that 13% of genetic diversity was explained by breed differences, whereas 27% and 60% came from among and within individuals respectively. The highest values of genetic diversity parameters including Na(7.29±0.29), Ne(5.73±0.28), Ho(0.74±0.05) and He(0.82±0.01) were observed for Morna, whereas their lowest values were found for Topras. However, the highest value of inbreeding coefficient (Fis) was found for Baluchi and the lowest for KB horses. Among the markers, CA425 was found as the most polymorphic and ASB23 as the least polymorphic and highly fixed marker. Results of structure analysis revealed that, except Topras, each local horse breed had very admixed genetic structure perhaps due to their continuous crossing with other breeds in the past. Moreover, the structure analysis also showed that Morna and Shiean breeds had mixing of each other which was also confirmed by the lowest value of their pairwise Fst values and likewise the phylogenetic analysis also showed their close genetic relationship with each other. The phylogenetic analysis also revealed that Shiean breed had close genetic relationship with Arabian horses. Collectively, our data showed that Morna is the largest and genetically most diverse horse breed of Pakistan, whereas Baluchi horses are the smallest in size and have the highest values of inbreeding coefficient. And the phylogeny analysis showed that Shiean breed had close genetic relationship with the Arabian.

Keywords: Biometric measurement; Evolutionary relationship; Genetic diversity and admixture; Indigenous horse breeds; Pakistan.