The clinical predictive value and regulation mechanism of microRNA-188-5p in contrast-induced acute kidney injury

Ran Yu; Caixia Wu; Yao Xiao; Qingju Li; Jiajia Chen; Jian Song; Haoyu Chen; Zhengqian Wang; Wanpeng Wang

doi:10.1016/j.bbrc.2023.09.019

The clinical predictive value and regulation mechanism of microRNA-188-5p in contrast-induced acute kidney injury

Biochem Biophys Res Commun. 2023 Oct 30:679:215-223. doi: 10.1016/j.bbrc.2023.09.019. Epub 2023 Sep 10.

Authors

Ran Yu¹, Caixia Wu², Yao Xiao³, Qingju Li¹, Jiajia Chen³, Jian Song², Haoyu Chen², Zhengqian Wang⁴, Wanpeng Wang⁵

Affiliations

¹ Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an, Jiangsu, 223400, PR China; School of Clinical Medicine, Medical College of Yangzhou University, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, Jiangsu, 225009, PR China; Jiangsu College of Nursing, Huai'an, Jiangsu, 223400, PR China.
² Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an, Jiangsu, 223400, PR China.
³ Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an, Jiangsu, 223400, PR China; Jiangsu College of Nursing, Huai'an, Jiangsu, 223400, PR China.
⁴ Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an, Jiangsu, 223400, PR China. Electronic address: 529318525@qq.com.
⁵ Lianshui People's Hospital, Kangda College of Nanjing Medical University, Huai'an, Jiangsu, 223400, PR China; School of Clinical Medicine, Medical College of Yangzhou University, Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou, Jiangsu, 225009, PR China; Jiangsu College of Nursing, Huai'an, Jiangsu, 223400, PR China. Electronic address: wangwanpeng123@163.com.

PMID: 37713958
DOI: 10.1016/j.bbrc.2023.09.019

Abstract

Contrast-induced acute kidney injury (CI-AKI), also known as contrast-induced nephropathy (CIN), has become the third leading cause of iatrogenic AKI. Serum creatinine (Scr) is currently used in CIN clinical diagnosis. Patients with increased Scr have developed severe kidney injury, so there is an urgent need to find a bio-marker for CIN early diagnosis. To investigate the changes in circulating microRNA-188-5p (miR-188-5p) after coronary angiography and its predictive value for the CIN occurrence, miR-188-5p expression in CIN rats from the GEO database and CIN patients and control patients from Lianshui People's Hospital was analyzed. The results showed that miR-188-5p expression in plasma and renal was higher in CIN group than in control group. Further, a total of 36 CIN patients and 108 non-CIN patients were included. There were significant differences in age, hypertension, diabetes, and contrast agent dosage. After 12 h of contrast agent application, circulating miR-188-5p expression in CIN group was higher than control group. Univariate and multivariate logistic regression analysis showed that age, hypertension, diabetes, contrast media dosage and postoperative miR-188-5p expression were closely related to CIN occurrence. For in vitro experiments, intracellular miR-188-5p expression was decreased with ioversol treatment, while miR-188-5p expression in supernatant was increased. To explore the potential mechanism of miR-188-5p in CIN, HK-2 cells were treated with NC mimic, ioversol, or miR-188-5p mimic. The results showed that the application of miR-188-5p mimic reduced apoptosis, reactive oxygen species and MDA, enhanced SOD and GSH contents. Further, it was confirmed that mRNA and protein levels of PTEN were up-regulated in ioversol-treated HK-2 cells, and down-regulated after miR-188-5p administration. Dual-luciferase reporter gene assay confirmed that PTEN was direct target gene of miR-188-5p. Above results suggest that circulating miR-188-5p has the potential to serve as a predictor of CIN.

Keywords: Biomarker; Contrast-induced nephropathy (CIN); Ferroptosis; Phosphatase and tensin homolog (PTEN); microRNA-188-5p (miR-188-5p).