Immuno-protective effect of neuropeptide Y immersion on the juvenile tilapia infected by Streptococcus agalactiae

Yang Yu; Ruoyun Li; Xiaozheng Yu; Yongqi Hu; Zongzhen Liao; Wensheng Li

doi:10.1016/j.fsi.2023.109072

Immuno-protective effect of neuropeptide Y immersion on the juvenile tilapia infected by Streptococcus agalactiae

Fish Shellfish Immunol. 2023 Oct:141:109072. doi: 10.1016/j.fsi.2023.109072. Epub 2023 Sep 12.

Authors

Yang Yu¹, Ruoyun Li², Xiaozheng Yu³, Yongqi Hu⁴, Zongzhen Liao⁵, Wensheng Li⁶

Affiliations

¹ State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: yuyang65@mail2.sysu.edu.cn.
² State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: 1048174623@qq.com.
³ State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: 15058173090@163.com.
⁴ State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: hyq133@163.com.
⁵ State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: liaozzh@mail2.sysu.edu.cn.
⁶ State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Aquatic Economic Animals, Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: lsslws@mail.sysu.edu.cn.

PMID: 37709180
DOI: 10.1016/j.fsi.2023.109072

Abstract

Neuropeptide Y (NPY), an important neurotransmitter, is widely distributed in the nervous systems of vertebrates. Multiple functions of NPY in mammals include the regulation of brain activity, emotion, stress response, feeding, digestion, metabolism and immune function. In the present study, we used synthetic NPY to immerse juvenile tilapia, thus firstly exploring the dose and time effect of this immersion. The results showed that the expression level of y8b and serum glucose increased after NPY immersion. When juvenile tilapia was challenged with Streptococcus agalactiae (S. agalactiae), no matter before or after the administration of NPY-immersion, it was found that NPY immersion could inhibit the expression of il-1β induced by S. agalactiae in telencephalon, hypothalamus, spleen and head kidney, and then promote the expression of il-10. In addition, NPY-immersion could reduce the activity of serum SOD but increase that of lysozyme, and ameliorate tissue damage in the head kidney and spleen of juvenile tilapia caused by S. agalactiae infection. This study firstly proposes the potential of NPY to be an immune protect factor in juvenile fish, and the results can provide a reference for the application of immersion administration in the immune protection of juvenile fish.

Keywords: Immersion; Immune protection; Neuropeptide Y; Streptococcus agalactiae; Tilapia.