Protoplast transient expression-based RNA-sequencing: A simple method to screen transcriptional regulation in plants

Jiameng Zhu; Suzhen Li; Haiyang Jiang; Di Lv; Shuai Ma; Baobao Wang; Xiangyu Lu; Wenzhu Yang; Rumei Chen; Xiaojin Zhou

doi:10.1093/plphys/kiad495

Protoplast transient expression-based RNA-sequencing: A simple method to screen transcriptional regulation in plants

Plant Physiol. 2023 Dec 30;194(1):408-411. doi: 10.1093/plphys/kiad495.

Authors

Jiameng Zhu^{1

2}, Suzhen Li¹, Haiyang Jiang², Di Lv¹, Shuai Ma^{1

3}, Baobao Wang¹, Xiangyu Lu¹, Wenzhu Yang¹, Rumei Chen¹, Xiaojin Zhou¹

Affiliations

¹ Crop Functional Genome Research Center, Chinese Academy of Agricultural Sciences, Biotechnology Research Institute, Beijing 100081, China.
² National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.
³ Chinese Academy of Agricultural Sciences, Institute of Crop Sciences, Beijing 100081, China.

Abstract

Protoplast transient expression-based RNA-sequencing identifies Opaque2 targets supported by molecular evidence and expression quantitative trait loci.

MeSH terms

DNA-Binding Proteins / metabolism
Gene Expression Regulation, Plant
Plants / metabolism
Protoplasts* / metabolism
RNA
Transcription Factors* / metabolism
Zea mays / genetics

Substances

Transcription Factors
DNA-Binding Proteins
RNA

Abstract

MeSH terms

Substances

Grants and funding