Discovery of multipotent progenitor cells from human induced membrane: Equivalent to periosteum-derived stem cells in bone regeneration

Hongri Wu; Jiulin Tan; Dong Sun; Xiaohua Wang; Jie Shen; Shulin Wang; Qijie Dai; Zhiyuan Wei; Gang Li; Sien Lin; Fei Luo; Zhao Xie

doi:10.1016/j.jot.2023.07.004

Discovery of multipotent progenitor cells from human induced membrane: Equivalent to periosteum-derived stem cells in bone regeneration

J Orthop Translat. 2023 Aug 30:42:82-93. doi: 10.1016/j.jot.2023.07.004. eCollection 2023 Sep.

Authors

Hongri Wu^{1

2}, Jiulin Tan¹, Dong Sun¹, Xiaohua Wang¹, Jie Shen¹, Shulin Wang¹, Qijie Dai¹, Zhiyuan Wei¹, Gang Li^{3

4

5}, Sien Lin^{3

4

5}, Fei Luo¹, Zhao Xie¹

Affiliations

¹ Department of Orthopaedics, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, PR China.
² Department of Orthopaedics, Navy 905 Hospital, Navy Medical University, Shanghai, PR China.
³ Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, PR China.
⁴ Musculoskeletal Research Laboratory, Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, PR China.
⁵ The CUHK-ACC Space Medicine Centre on Health Maintenance of Musculoskeletal System, The Chinese University of Hong Kong Shenzhen Research Institute, Shenzhen, PR China.

Abstract

Background: The periosteum stem cells (PSCs) plays a critical role in bone regeneration and defect reconstruction. Insertion of polymethyl methacrylate (PMMA) bone cement can form an induced membrane(IM) and showed promising strategy for bone defect reconstruction, the underlying mechanism remains unclear. Our study sought to determine whether IM-derived cells(IMDCs) versus PSCs have similar characteristics in bone regeneration.

Methods: IM and periosteum were harvested from ten bone defect patients treated with PMMA, the IMDCs and PSCs were isolated respectively. Morphological, functional and molecular evaluation was performed and matched for comparison.

Results: Both progenitor-like IMDCs and PSCs were successfully isolated. In vitro, we found IMDCs were similar to PSCs in morphology, colony forming capacity and expression of surface marker(CD90⁺, CD73⁺, CD105⁺, CD34^-/CD45^-). Meanwhile, these IMSCs displayed multipotency with chondrogenic, adipogenic and osteogenic differentiation, but differed in some IMSCs(3/10) population showing relatively poor osteogenic differentiation. The molecular profiles suggests that cell cycle and DNA replication signaling pathways were associated with these varying osteogenic potential. In vivo, we established a cell-based tissue-engineered bone by seeding IMDSs/PSCs to demineralized bone matrix (DBM) scaffold and demonstrated both IMDSs and PSCs enhanced bone regeneration in SCID mice bone defect model compared with DBM alone.

Conclusion: Our data demonstrated IM containing multipotent progenitor cells similar to that periosteum promoting bone regeneration, and indicated the existence of multiple subsets in osteogenic differentiation. Overall, the study provided a cellular and molecular insights in understanding the successful or failed outcome of bone defect healing.The translational potential of this article: This study confirmed IMDCs and PSCs share similar regeneration capacity and inform a translation potential of that cellular therapy applying IMDCs in bone defect repair.

Keywords: Bone regeneration; Differentiation; Induced membrane; Periosteum; Stem cell.