Oxidized of chitosan with different molecular weights for potential antifungal and plant growth regulator applications

Haoyang Du; Fengkun Gao; Shu Yang; Hongxia Zhu; Caihong Cheng; Fei Peng; Wenjing Zhang; Zhe Zheng; Xiuping Wang; Yuedong Yang; Wenlong Hou

doi:10.1016/j.ijbiomac.2023.126862

Oxidized of chitosan with different molecular weights for potential antifungal and plant growth regulator applications

Int J Biol Macromol. 2023 Dec 31;253(Pt 3):126862. doi: 10.1016/j.ijbiomac.2023.126862. Epub 2023 Sep 11.

Authors

Haoyang Du¹, Fengkun Gao¹, Shu Yang², Hongxia Zhu³, Caihong Cheng⁴, Fei Peng⁴, Wenjing Zhang¹, Zhe Zheng¹, Xiuping Wang⁵, Yuedong Yang⁶, Wenlong Hou⁷

Affiliations

¹ Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; College of Chemical Engineering, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.
² College of Chemical Engineering, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.
³ College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, PR China.
⁴ Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Analysis and Testing Center, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China.
⁵ Analysis and Testing Center, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China. Electronic address: wangxiuping0721@163.com.
⁶ Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China. Electronic address: yuedongyang@hotmail.com.
⁷ Hebei Key Laboratory of Active Components and Functions in Natural Products, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; Analysis and Testing Center, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China. Electronic address: wenlonghou@126.com.

PMID: 37703971
DOI: 10.1016/j.ijbiomac.2023.126862

Abstract

The application of Chitosan (CS) in drug delivery systems, plant growth promotion, antibacterial potentiality and plant defense is significantly limited by its inability to dissolve in neutral solutions. In this work, CS with different molecular weights (M_w) has been oxidized, yielding five kinds of oxidized chitosan (OCS 1-5) with solubilities in neutral solutions. The results obtained from Fourier Transform Infrared Spectroscopy clearly showed the successful oxidation of the hydroxyl group to form aldehyde and carboxyl groups. And the CS derivatives showed the wrinkled and lamellar structures on the surface of OCS. The results of antifungal activity against Fusarium graminearum showed that the OCS dissolved in 2 % (V/V) acetic acid exhibited better performance of almost complete inhibition of mycelial growth compared with CS at the concentration of 500 μg/mL. Among the five OCS, OCS-4 exhibited the best antifungal effect and had the lowest EC₅₀ value of 581.68 μg/mL in samples. OCS-4 displayed superior promoting effect on seed germination with a germination potential of 62.2 % at a concentration of 3 g/L and a germination rate of 74.5 %. Additionally, the other four OCS also showed excellent antifungal activity with dose-dependent manners. These results indicated that the OCS had excellent antifungal potential in agricultural production.

Keywords: Antifungal; Chitosan; Oxidized chitosan.

MeSH terms

Antifungal Agents* / chemistry
Antifungal Agents* / pharmacology
Chitosan* / chemistry
Molecular Weight
Oxidation-Reduction
Plant Growth Regulators
Spectroscopy, Fourier Transform Infrared

Substances

Antifungal Agents
Chitosan
Plant Growth Regulators