ISSCR standards for the use of human stem cells in basic research

Tenneille E Ludwig; Peter W Andrews; Ivana Barbaric; Nissim Benvenisty; Anita Bhattacharyya; Jeremy M Crook; Laurence M Daheron; Jonathan S Draper; Lyn E Healy; Meritxell Huch; Maneesha S Inamdar; Kim B Jensen; Andreas Kurtz; Madeline A Lancaster; Prisca Liberali; Matthias P Lutolf; Christine L Mummery; Martin F Pera; Yoji Sato; Noriko Shimasaki; Austin G Smith; Jihwan Song; Claudia Spits; Glyn Stacey; Christine A Wells; Tongbiao Zhao; Jack T Mosher

doi:10.1016/j.stemcr.2023.08.003

ISSCR standards for the use of human stem cells in basic research

Stem Cell Reports. 2023 Sep 12;18(9):1744-1752. doi: 10.1016/j.stemcr.2023.08.003.

Authors

Tenneille E Ludwig¹, Peter W Andrews², Ivana Barbaric², Nissim Benvenisty³, Anita Bhattacharyya⁴, Jeremy M Crook⁵, Laurence M Daheron⁶, Jonathan S Draper⁷, Lyn E Healy⁸, Meritxell Huch⁹, Maneesha S Inamdar¹⁰, Kim B Jensen¹¹, Andreas Kurtz¹², Madeline A Lancaster¹³, Prisca Liberali¹⁴, Matthias P Lutolf¹⁵, Christine L Mummery¹⁶, Martin F Pera¹⁷, Yoji Sato¹⁸, Noriko Shimasaki¹⁹, Austin G Smith²⁰, Jihwan Song²¹, Claudia Spits²², Glyn Stacey²³, Christine A Wells²⁴, Tongbiao Zhao²⁵, Jack T Mosher²⁶

Affiliations

¹ WiCell Research Institute, Madison, WI, USA. Electronic address: tludwig@wicell.org.
² The University of Sheffield, Sheffield, UK.
³ Hebrew University, Jerusalem, Israel.
⁴ University of Wisconsin-Madison, Madison, WI, USA.
⁵ The University of Sydney, Camperdown, NSW Australia; Chris O'Brien Lifehouse, Camperdown, NSW, Australia; The University of Wollongong, Wollongong, NSW, Australia.
⁶ Harvard Stem Cell Institute, Boston, MA, USA.
⁷ Stem Cell Network, Ottawa, ON, Canada.
⁸ Francis Crick Institute, London, UK.
⁹ Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
¹⁰ Jawaharlal Nehru Centre for Advanced Scientific Research, Institute for Stem Cell Science and Regenerative Medicine, Bangalore, Karnataka, India.
¹¹ Novo Nordisk Foundation Center for Stem Cell Medicine, University of Copenhagen, Copenhagen, Denmark.
¹² Fraunhofer Institute for Biomedical Engineering, Sulzbach, Germany; Berlin Institute of Health at Charité, Berlin, Germany.
¹³ MRC Laboratory of Molecular Biology, Cambridge, UK.
¹⁴ Friedrich Miescher Institute for Biomedical Research, Basal, Switzerland.
¹⁵ Swiss Federal Institute of Technology (EPFL), Basel, Switzerland.
¹⁶ Leiden University Medical Center, Leiden, the Netherlands.
¹⁷ The Jackson Laboratory, Bar Harbor, ME, USA.
¹⁸ National Institute of Health Sciences, Kawasaki, Japan.
¹⁹ Center for iPS Research and Application, Kyoto, Japan; Prefectural University of Medicine, Nagoya University, Nagoya, Japan; National University of Singapore, Singapore, Singapore.
²⁰ University of Exeter, Exeter, UK.
²¹ CHA University, Seoul, Korea; iPS Bio, Inc, Seoul, Korea.
²² Vrije Universiteit Brussel, Brussels, Belgium.
²³ International Stem Cell Banking Initiative, Barley, Herts, UK.
²⁴ University of Melbourne, Melbourne, VIC, Australia.
²⁵ Institute of Zoology Chinese Academy of Sciences, Beijing Institute for Stem Cell and Regenerative Medicine, Beijing, China.
²⁶ International Society for Stem Cell Research, Evanston, IL, USA.

Abstract

The laboratory culture of human stem cells seeks to capture a cellular state as an in vitro surrogate of a biological system. For the results and outputs from this research to be accurate, meaningful, and durable, standards that ensure reproducibility and reliability of the data should be applied. Although such standards have been previously proposed for repositories and distribution centers, no widely accepted best practices exist for laboratory research with human pluripotent and tissue stem cells. To fill that void, the International Society for Stem Cell Research has developed a set of recommendations, including reporting criteria, for scientists in basic research laboratories. These criteria are designed to be technically and financially feasible and, when implemented, enhance the reproducibility and rigor of stem cell research.

Keywords: hESCS; hIPSCs; human; model systems; organoids; tissue stem cells.

ISSCR standards for the use of human stem cells in basic research

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