Photostable yellow emissive carbon dots for iron-mediated reversible sensing of biothiols and cellular imaging

Abstract

The different levels of biothiols in cells can not only screen cancer cells but also play a selective role in killing cancer cells. Therefore, accurate monitoring of biothiol in cancer cells is of great research significance. Herein, novel yellow emission CDs (Y-CDs) were prepared by a simple hydrothermal method using 2, 5-dihydroxyterephthalic acid (DHTA) as precursors. The Y-CDs as a highly efficient dual-mode sensor could detect Fe³⁺ and biothiols by colorimetric and fluorescence signals. Especially, with the addition of L-Cysteine, the quenched fluorescence could be quickly restored within 2 min and the detection limit was as low as 31.65 nM. Additionally, this sensor was utilized to sense biothiols in actual samples and living cells due to its eminent biocompatibility. Finally, the Y-CDs were successfully applied not only as fluorescent ink for message encryption but also as a portable solid hydrogels sensor for the detection of Fe³⁺ and biothiols. Therefore, these results suggested that Y-CDs could serve as a promising sensor for Fe³⁺ and biothiol detection in early cancer screening.

Keywords: Biothiol; Colorimetric; Fe(3+); Fluorescent; Living cells; Sensor.