Wastewater discharge is considered to be one of the anthropogenic factors affecting the water quality of urban rivers. The source and composition of wastewater are complex and diverse, and it is difficult to evaluate its effect on water quality and ecological health of receiving waters. Environmental DNA method can determine all species living in waters by examining DNA sequences, reflecting the impact of water quality changes on aquatic systems. In this study, water samples from two urban rivers were collected in dry and wet seasons, and the composition of pollutants was investigated by nontarget screening. Based on the pollutant composition, compound toxicity prediction and concentration addition model were used to predict the toxicity changes of pollutants in the urban rivers. More than 1500 suspect organic pollutants were nontarget screened, and silafluofen was found to be a major toxicity contributor. Environmental DNA analysis was combined with water quality measure and pollutant toxicity prediction to reveal the effects of pollutants from different sources on aquatic ecosystems. Fish diversity was negatively correlated with the mixed toxicity of organic pollutants, suggesting potential ecological risk in these two urban rivers. Our study developed a water quality assessment method based on pollutant composition and toxicity, and the potential risk of nonpoint source pollutants on aquatic ecosystems should not be neglected.
Keywords: Environmental DNA; Nontarget substance screening; Pesticides; Pollutant composition; Predicted toxicity.
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