Dieting alleviates hyperuricemia and organ injuries in uricase-deficient rats via down-regulating cell cycle pathway

PeerJ. 2023 Sep 8:11:e15999. doi: 10.7717/peerj.15999. eCollection 2023.

Abstract

Dieting is a basic treatment for lowering hyperuricemia. Here, we aimed to determine the optimal amount of dietary food that lowers serum uric acid (SUA) without modifying the dietary ingredients in rats. Increased SUA was found in food-deprived 45-day-old uricase-deficient rats (Kunming-DY rats), and the optimal amount of dietary food (75% dietary intake) to lower SUA was established by controlling the amount of food given daily from 25% to 100% for 2 weeks. In addition to lowering SUA by approximately 22.5 ± 20.5%, the optimal amount of dietary food given for 2 weeks inhibited urine uric acid excretion, lowered the uric acid content in multiple organs, improved renal function, lowered serum triglyceride, alleviated organ injuries (e.g., liver, kidney and intestinal tract) at the histological level, and down-regulated the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway of the cell cycle (ko04110). Taken together, these results demonstrate that 75% dietary food effectively lowers the SUA level without modifying dietary ingredients and alleviates the injuries resulting from uricase deficiency or hyperuricemia, the mechanism of which is associated with the down-regulation of the cell cycle pathway.

Keywords: Cell cycle pathway; Dieting; Fasting; Hyperuricemia; Renal injury; Serum uric acid; Uricase-deficient rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle
  • Cell Division
  • Hyperuricemia*
  • Pharmaceutical Vehicles
  • Rats
  • Urate Oxidase
  • Uric Acid

Substances

  • Urate Oxidase
  • Uric Acid
  • Pharmaceutical Vehicles

Grants and funding

The work was financially supported by the National Natural Science Foundation of China (No. 82260886), the Yunnan Provincial Science and Technology Department–Applied Basic Research Joint Special Funds of Kunming Medical University (202101AY070001-007), and the Yunnan Provincial Science and Technology Department–Applied Basic Research Joint Special Funds of Yunnan University of Traditional Chinese Medicine (202101AZ070001-010, 202101AZ070001-093, 202101AZ070001-242). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.