Genome-wide QTL mapping for stripe rust resistance in spring wheat line PI 660122 using the Wheat 15K SNP array

Qiong Yan; Guoyun Jia; Wenjing Tan; Ran Tian; Xiaochen Zheng; Junming Feng; Xiaoqin Luo; Binfan Si; Xin Li; Kebing Huang; Meinan Wang; Xianming Chen; Yong Ren; Suizhuang Yang; Xinli Zhou

doi:10.3389/fpls.2023.1232897

Genome-wide QTL mapping for stripe rust resistance in spring wheat line PI 660122 using the Wheat 15K SNP array

Front Plant Sci. 2023 Aug 28:14:1232897. doi: 10.3389/fpls.2023.1232897. eCollection 2023.

Authors

Qiong Yan¹, Guoyun Jia¹, Wenjing Tan¹, Ran Tian¹, Xiaochen Zheng¹, Junming Feng¹, Xiaoqin Luo¹, Binfan Si¹, Xin Li¹, Kebing Huang¹, Meinan Wang², Xianming Chen^{2

3}, Yong Ren⁴, Suizhuang Yang¹, Xinli Zhou¹

Affiliations

¹ Wheat Research Institute, School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, China.
² Department of Plant Pathology, Washington State University, Pullman, WA, United States.
³ Wheat Health, Genetics, and Quality Research Unit, US Department of Agriculture-Agricultural Research Service (USDA-ARS), Pullman, WA, United States.
⁴ Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang Institute of Agricultural Science, Mianyang, Sichuan, China.

Abstract

Introduction: Stripe rust is a global disease of wheat. Identification of new resistance genes is key to developing and growing resistant varieties for control of the disease. Wheat line PI 660122 has exhibited a high level of stripe rust resistance for over a decade. However, the genetics of stripe rust resistance in this line has not been studied. A set of 239 recombinant inbred lines (RILs) was developed from a cross between PI 660122 and an elite Chinese cultivar Zhengmai 9023.

Methods: The RIL population was phenotyped for stripe rust response in three field environments and genotyped with the Wheat 15K single-nucleotide polymorphism (SNP) array.

Results: A total of nine quantitative trait loci (QTLs) for stripe rust resistance were mapped to chromosomes 1B (one QTL), 2B (one QTL), 4B (two QTLs), 4D (two QTLs), 6A (one QTL), 6D (one QTL), and 7D (one QTL), of which seven QTLs were stable and designated as QYrPI660122.swust-4BS, QYrPI660122.swust-4BL, QYrPI660122.swust-4DS, QYrPI660122.swust-4DL, QYrZM9023.swust-6AS, QYrZM9023.swust-6DS, and QYrPI660122.swust-7DS. QYrPI660122.swust-4DS was a major all-stage resistance QTL explaining the highest percentage (10.67%-20.97%) of the total phenotypic variation and was mapped to a 12.15-cM interval flanked by SNP markers AX-110046962 and AX-111093894 on chromosome 4DS.

Discussion: The QTL and their linked SNP markers in this study can be used in wheat breeding to improve resistance to stripe rust. In addition, 26 lines were selected based on stripe rust resistance and agronomic traits in the field for further selection and release of new cultivars.

Keywords: QTL mapping; resistance; stripe rust; wheat; yellow rust.

Grants and funding

This study was financially supported by the Breakthrough in Wheat Breeding Material and Method Innovation and New Variety Breeding (Breeding Research Project, 2021YFYZ0002) and was partially funded by the Key Research and Development Program of the International Science and Technology Innovation Cooperation of Science and Technology Department of Sichuan Province, China (No. 2022YFH0032), National Natural Science Foundation of China (No. 32101707), PhD Foundation of Southwest University of Science and Technology (Nos. 19zx7116, 18zx7159, 16zx7162), and Longshan Academic Talent Research Support Program of SWUST (No. 17LZX5).