The green bio-manufacturing industry, characterized with high efficiency, safety, energy-saving, and environmental-friendliness, is a national strategic emerging industry with broad market prospect. Industrial enzyme is the "chip" of green biological process. The exploitation and application of new industrial enzymes is one of the core enabling technologies of green bio-manufacturing. This review introduces the current situation of industrial enzyme industry, followed by summarizing a series of key technical breakthroughs and research progress in industrial enzymes as well as green biological technologies and processes, which were developed by Tianjin institute of industrial biotechnology, Chinese Academy of Sciences in the past 10 years. Typical cases where traditional processing industry was promoted by the development and application of enzyme and green biological technologies were also presented. It is envisioned that development of these core technologies will enable more traditional processing industries transform into green and sustainable bio-based industry.
Keywords: directed evolution; expression system; gene mining; green bio-manufacturing; high-throughput screening; industrial enzyme; protein rational design.