Relative contribution of canopy and soil effects between plants with different metal tolerance along a metal pollution gradient

Abstract

Multiple effects, operating either on the long-term (soil-engineering effects) or on the short-term during plant life (microclimate modification or resources pre-emption), can act simultaneously and determine the outcome of plant-plant interactions. These diverse effects have not been disentangled along a gradient of metal/metalloid pollution, although this is crucial for understanding the dominant species turnover along the gradient, and thus the driving processes of facilitation recurrently found in metalliferous ecosystems, which could help improving ecological restoration of these degraded ecosystems. Here, we experimentally assessed different short-term effects of two dominant forbs of highly polluted habitats (Hutchinsia alpina and Arenaria multicaulis, tolerant to metal stress) and two grasses of less polluted habitats (Agrostis capillaris and Festuca rubra, less tolerant to metal stress) on target plant species (the same as the dominant species mentioned above) transplanted along a large metal pollution gradient. Additionally, in highly polluted environments, we differentiated short- from long-term effects of the two metallicolous forbs, which had different abilities to concentrate metals in their leaves. In line with other studies along metal gradients, variation of short-term interactions appeared to follow the Stress Gradient Hypothesis for plants less adapted to metal pollution (p = 0.030), with positive interactions dominating in most severe areas. Regarding long-term effects, the species with highest leaf metal-accumulation showed no negative effect contrary to the Elemental allelopathy Hypothesis. Long-term effects of the species with lower leaf-metal accumulation could not be determined because of the occurrence of an unexpected difference in micro-habitat conditions (soil depth and humidity) for this species along the metal pollution gradient. Increasing short-term facilitation along metal pollution gradients, which confirmed previous studies, is promising for improving conditions and restoring the most polluted environments. However, long-term results stressed the difficulty to quantify these effects given that these areas are highly fragmented and heterogeneous.

Keywords: Ecological restoration; Elemental allelopathy hypothesis; Habitat heterogeneities; Metals and metalloids; Stress gradient hypothesis; Stress tolerance.